Tomusiak-Plebanek Anna, Heczko Piotr, Skowron Beata, Baranowska Agnieszka, Okoń Krzysztof, Thor Piotr J, Strus Magdalena
Department of Microbiology, Jagiellonian University Medical College, Poland,
Department of Pathophysiology, Jagiellonian University Medical College, Poland.
Drug Des Devel Ther. 2018 Sep 28;12:3221-3233. doi: 10.2147/DDDT.S164559. eCollection 2018.
Some lactobacilli, which possess superoxide dismutase-like activity and catalase activity naturally, have strong antioxidative properties. The aim of this study was to identify such strains and check which of them play a crucial role in alleviating intestinal inflammation.
We selected two strains for use in animal studies: 30B (which has the highest catalase activity) and 900 (which has the highest dismutase-like activity). Forty mice (C57B1/6J) were divided into four experimental groups with ten mice in each group. Group I (control group) was not supplemented with , group II (catalase group) was orally supplemented with 30B, group III (dismutase-like group) was supplemented with 900, and group IV (mixed group) was supplemented with both strains. For 23 days, the temperature and body mass of each mouse were recorded and fecal samples for microbiological examination were collected. On day 23, the animals were sacrificed, and their intestines were removed for microbiological and histopathological studies.
Compared to the control group, the highest drop in the body temperature was observed in groups II (<0.05) and IV (<0.05). Similarly, groups II (<0.05) and IV (<0.05) had the highest drop in body mass. Moreover, histopathological evaluation of colon fragments showed intracryptic abscesses in these groups. Group III mice showed most limited degree of inflammation.
strains with dismutase-like activity are more effective in alleviating intestinal inflammation than strains producing catalase, suggesting that superoxide anion radical decomposition is crucial in this process.
一些天然具有超氧化物歧化酶样活性和过氧化氢酶活性的乳酸菌具有很强的抗氧化特性。本研究的目的是鉴定这些菌株，并检查其中哪些在减轻肠道炎症中起关键作用。
我们选择了两株用于动物研究的菌株：30B（过氧化氢酶活性最高）和900（超氧化物歧化酶样活性最高）。40只小鼠（C57B1/6J）被分为四个实验组，每组10只小鼠。第一组（对照组）不补充任何物质，第二组（过氧化氢酶组）口服补充30B，第三组（超氧化物歧化酶样组）补充900，第四组（混合组）补充两种菌株。在23天内，记录每只小鼠的体温和体重，并收集粪便样本进行微生物学检查。在第23天，处死动物，取出它们的肠道进行微生物学和组织病理学研究。
与对照组相比，第二组（<0.05）和第四组（<0.05）的体温下降幅度最大。同样，第二组（<0.05）和第四组（<0.05）的体重下降幅度最大。此外，结肠片段的组织病理学评估显示这些组存在隐窝内脓肿。第三组小鼠的炎症程度最有限。
具有超氧化物歧化酶样活性的菌株在减轻肠道炎症方面比产生过氧化氢酶的菌株更有效，这表明超氧阴离子自由基的分解在这个过程中至关重要。
