Gérard Davy, Wenger Jérôme, Devilez Alexis, Gachet David, Stout Brian, Bonod Nicolas, Popov Evgeny, Rigneault Hervé
Institut Fresnel, Aix-Marseille Université, CNRS, 13397 Marseille, France.
Opt Express. 2008 Sep 15;16(19):15297-303. doi: 10.1364/oe.16.015297.
Latex microspheres are used as a simple and low-cost means to achieve three axis electromagnetic confinement below the standard diffraction limit. We demonstrate their use to enhance the fluorescence fluctuation detection of single molecules. Compared to confocal microscopy with high numerical aperture, we monitor a detection volume reduction of one order of magnitude below the diffraction limit together with a 5-fold gain in the fluorescence rate per molecule. This offers new opportunities for a broad range of applications in biophotonics, plasmonics, optical data storage and ultramicroscopy.
乳胶微球被用作一种简单且低成本的手段，以在标准衍射极限以下实现三轴电磁限制。我们展示了它们用于增强单分子荧光涨落检测的用途。与具有高数值孔径的共聚焦显微镜相比，我们监测到检测体积在衍射极限以下减小了一个数量级，同时每个分子的荧光速率提高了5倍。这为生物光子学、等离子体学、光学数据存储和超显微镜等广泛应用提供了新机会。
