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[HPLC method for determination of vitamin C in milk, whey and whey beverages].

作者信息

Kneifel W, Sommer R

出版信息

Z Lebensm Unters Forsch. 1985 Aug;181(2):107-10. doi: 10.1007/BF01042570.

DOI:10.1007/BF01042570
PMID:4050123
Abstract

A method is described for the estimation of L-ascorbic acid and L-dehydroascorbic acid in milk, whey, and whey drinks by paired-ion reversed-phase HPLC using a C18-column. L-ascorbic acid is detected UV-spectrophotometrically at 248 nm, L-dehydroascorbic acid is determined spectrofluorometrically with excitation at 350 nm and emission at 430 nm, after derivatisation into a quinoxaline fluorophor.

摘要

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[HPLC method for determination of vitamin C in milk, whey and whey beverages].
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Precision of dehydroascorbic acid quantitation with the use of the subtraction method--validation of HPLC-DAD method for determination of total vitamin C in food.使用减法法测定脱氢抗坏血酸的精密度——HPLC-DAD 法测定食品中总维生素 C 的验证。
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