Reich J D, Cazzaniga A L, Mertz P M, Kerdel F A, Eaglstein W H
Department of Dermatology and Cutaneous Surgery, University of Miami School of Medicine, FL 33101.
J Am Acad Dermatol. 1991 Jul;25(1 Pt 1):40-6. doi: 10.1016/0190-9622(91)70171-w.
Many cutaneous disorders are associated with activation or increased numbers of mast cells. Electrical stimulation has been shown to be effective in treating many of these disorders. This study is designed to examine the effect of electrical stimulation on mast cells in acute wounds. Four pathogen-free pigs received 20 wounds, each of which was subjected to biopsy at various times after wounding. Half of the wounds were treated with electrical stimulation and the other half were treated with a sham electrode. The biopsy specimens were fixed in Carnoy's medium and stained with alcian blue and Nuclear Fast Red. Mast cells from both sets of wounds were counted and analyzed. Highly significant reductions in the number of mast cells were seen with electrical stimulation on days 1 and 2 compared with nonstimulated control wounds. Electron microscopy was performed to compare the stimulated and control mast cells for characteristic features in morphology, location, and evidence of degranulation. Electrical stimulation did not appear to induce degranulation. The ability of electrical stimulation to decrease the number of mast cells may be related to a reduction of either proliferation or migration of these cells and may prove to be a valuable therapeutic technique.
许多皮肤疾病都与肥大细胞的激活或数量增加有关。电刺激已被证明对治疗其中许多疾病有效。本研究旨在探讨电刺激对急性伤口中肥大细胞的影响。四只无特定病原体猪接受了20处伤口，每处伤口在受伤后的不同时间进行活检。一半伤口接受电刺激治疗，另一半用假电极治疗。活检标本用卡诺伊氏固定液固定，并用阿尔辛蓝和核固红染色。对两组伤口的肥大细胞进行计数和分析。与未刺激的对照伤口相比，电刺激在第1天和第2天导致肥大细胞数量显著减少。进行电子显微镜检查以比较受刺激和对照肥大细胞在形态、位置和脱颗粒证据方面的特征。电刺激似乎不会诱导脱颗粒。电刺激减少肥大细胞数量的能力可能与这些细胞增殖或迁移的减少有关，可能是一种有价值的治疗技术。
