Alvarez O M, Mertz P M, Smerbeck R V, Eaglstein W H
J Invest Dermatol. 1983 Aug;81(2):144-8. doi: 10.1111/1523-1747.ep12543498.
We studied the effects of direct electric current supplied by an energized silver-coated electrode on dermal and epidermal wound healing. Keratome-induced wounds (0.3 mm deep) on the skin of young domestic pigs were treated with either an energized (50-300 microA) electrode (DC), an unenergized electrode (placebo), or left untreated. Wounds were excised on days 1-7 after wounding and the epidermis was separated from the dermis. The epidermal sheet was evaluated for reepithelialization and the dermis was assayed for collagen biosynthetic capacity. Dermal collagen production among treatments did not differ markedly on days 1-4 after wounding. However, a highly significant increase (p less than 0.001) in the collagen synthetic capacity was observed on days 5, 6, and 7 in wound treated with DC. There was no significant difference in collagen synthesis among treatments when collagen production was corrected for DNA content. The rate of wound epithelialization was also significantly accelerated (p less than 0.05) in DC-treated wounds. These results suggest that the proliferative and/or migratory capacity of epithelial and connective tissue cells involved in repair and regeneration can be affected by an electrical field.
我们研究了由通电的镀银电极提供的直流电对皮肤和表皮伤口愈合的影响。在幼年家猪皮肤上用角膜刀造成深度为0.3毫米的伤口，分别用通电电极（50 - 300微安）（直流电）、未通电电极（安慰剂）处理或不进行处理。在受伤后第1 - 7天切除伤口，将表皮与真皮分离。对表皮片进行再上皮化评估，对真皮进行胶原蛋白生物合成能力检测。在受伤后第1 - 4天，各处理组之间的真皮胶原蛋白生成没有明显差异。然而，在用直流电处理的伤口中，在第5、6和7天观察到胶原蛋白合成能力有极显著增加（p小于0.001）。当根据DNA含量校正胶原蛋白生成量时，各处理组之间的胶原蛋白合成没有显著差异。在直流电处理的伤口中，伤口上皮化速率也显著加快（p小于0.05）。这些结果表明，参与修复和再生的上皮和结缔组织细胞的增殖和/或迁移能力可能会受到电场的影响。
