Kondo S, Yamaguchi N, Imanishi T, Sakota H, Jokei Y, Mituda A
Department of Clinical Medicine, Kobe University School of Medicine.
Rinsho Byori. 1991 Jun;39(6):615-22.
Sysmex NE-7000 flagging system was evaluated for the recognition of abnormal cells in white cell differentials. Six flags that could mark blasts, immature granulocytes, left shift, nucleated red blood cells, atypical lymphocytes, and platelet clumps were selected for evaluation. 182 morphologic abnormal samples were screened from the Kobe University Hospital patients, by means of the 600 eye counts differentials. Blasts flag missed 8 of 28 cases, and immature granulocytes flag missed 25 of 56 cases. These missed cases contained either relatively few abnormal cells; less than 3% immature granulocytes, or absolutely few cells; less than 201 per micro liter blasts. Platelet clumps flag missed only 2 of 23 cases. But nucleated red blood cells and atypical lymphocytes flags missed all of 7 and 4 cases respectively. Left shift flag missed 36 of 64 cases, but it could help marking immature granulocytes or blasts. To use the NE-7000 as a screening tool, we compared the NE's all 35 flags versus abnormal cells or status based on the 100 eye counts. 1,153 cases were analyzed. Full agreement; 1047 (90.8%), partial agreement; 61 (5.2%), distributional false normal; 7 (1.6%), and morphological false normal; 4 (4.6%), were obtained. The more flags were used, the less abnormal cases were missed. But it accompanied the increase of the flag positive cases that must be confirmed by eye.
对Sysmex NE - 7000标记系统进行了评估，以识别白细胞分类中的异常细胞。选择了六个可标记原始细胞、未成熟粒细胞、核左移、有核红细胞、非典型淋巴细胞和血小板聚集的标记进行评估。通过600次镜检分类，从神户大学医院患者中筛选出182份形态学异常样本。原始细胞标记漏检了28例中的8例，未成熟粒细胞标记漏检了56例中的25例。这些漏检病例要么异常细胞相对较少；未成熟粒细胞少于3%，要么绝对细胞数量较少；每微升原始细胞少于201个。血小板聚集标记仅漏检了23例中的2例。但有核红细胞和非典型淋巴细胞标记分别漏检了所有7例和4例。核左移标记漏检了64例中的36例，但它有助于标记未成熟粒细胞或原始细胞。为了将NE - 7000用作筛选工具，我们基于100次镜检，将NE的所有35个标记与异常细胞或状态进行了比较。分析了1153例病例。得到完全一致的情况有1047例（90.8%），部分一致的有61例（5.2%），分布性假正常的有7例（1.6%），形态学假正常的有4例（4.6%）。使用的标记越多，漏检的异常病例就越少。但这伴随着需要镜检确认的标记阳性病例的增加。
