Feng J, Zhang M, Zheng S, Xie P, Ma A
Institute of Animal Science, Chinese Academy of Agricultural Science, State Key Laboratory of Animal Nutrition, Beijing, 100094, China.
Poult Sci. 2008 Oct;87(10):2133-9. doi: 10.3382/ps.2007-00358.
The effects of high temperature on multiple parameters of broilers were evaluated both in vitro and in vivo. In the in vitro study, the bilateral musculus fibularis longus muscles of 8 broilers were isolated and incubated at either 41.5 or 44.5 degrees C. The greater incubation temperature increased mitochondrial H2O2 production by 28.0% (P < 0.0001), malondialdehyde concentration by 16.8% (P = 0.0368), and lactate concentration by 33.0% (P < 0.0001) and decreased mitochondrial Ca2+-ATPase activity by 19.6% (P = 0.0001). In the in vivo study, 180 four-week-old broilers were kept in 3 controlled-environment chambers for 3 wk. High temperature increased mitochondrial H2O2 production (P < 0.05) in liver, malondialdehyde concentration in liver and breast muscle, and lactate concentration in breast muscle (P < 0.05). In addition, it inhibited mitochondrial Ca2+-ATPase activity in muscle and liver (P < 0.05). High temperature also significantly decreased initial pH and increased L*, drip loss, and shear force of broiler breast muscle.
在体外和体内评估了高温对肉鸡多个参数的影响。在体外研究中，分离出8只肉鸡的双侧腓骨长肌，并在41.5℃或44.5℃下孵育。较高的孵育温度使线粒体过氧化氢生成量增加28.0%（P<0.0001），丙二醛浓度增加16.8%（P=0.0368），乳酸浓度增加33.0%（P<0.0001），并使线粒体Ca2+-ATP酶活性降低19.6%（P=0.0001）。在体内研究中，将180只4周龄肉鸡饲养在3个可控环境舱中3周。高温增加了肝脏中线粒体过氧化氢的生成（P<0.05）、肝脏和胸肌中的丙二醛浓度以及胸肌中的乳酸浓度（P<0.05）。此外，它还抑制了肌肉和肝脏中线粒体Ca2+-ATP酶的活性（P<0.05）。高温还显著降低了肉鸡胸肌初始pH值，并增加了L*值、滴水损失和剪切力。
