Blancas Alicia A, Lauer Nicholas E, McCloskey Kara E
Graduate Program in Quantitative and Systems Biology, University of California at Merced, California, USA.
Curr Protoc Stem Cell Biol. 2008 Sep;Chapter 1:Unit 1F.5. doi: 10.1002/9780470151808.sc01f05s6.
Vascular progenitor cells derived from stem cells could potentially lead to a variety of clinically relevant applications, including cell-based therapies and tissue engineering. Here, we describe methods for isolating purified proliferating populations of vascular endothelial cells from mouse embryonic stem cells (mESC) using Flk-1 positive sorted cells, VEGF supplementation, and a rigorous manual selection technique required for endothelial cell purification and expansion. Using this in vitro derivation procedure, it is possible to obtain millions of cells at various stages of differentiation, with the potential for up to 25 population doublings.
源自干细胞的血管祖细胞可能会带来多种临床相关应用，包括基于细胞的疗法和组织工程。在此，我们描述了从小鼠胚胎干细胞（mESC）中分离纯化增殖性血管内皮细胞群体的方法，该方法使用Flk-1阳性分选细胞、补充血管内皮生长因子（VEGF），以及内皮细胞纯化和扩增所需的严格手动筛选技术。使用这种体外衍生程序，可以获得数百万处于不同分化阶段的细胞，其潜在的群体倍增次数可达25次。
