胚胎干细胞的内皮分化
Endothelial differentiation of embryonic stem cells.
作者信息
Blancas Alicia A, Lauer Nicholas E, McCloskey Kara E
机构信息
Graduate Program in Quantitative and Systems Biology, University of California at Merced, California, USA.
出版信息
Curr Protoc Stem Cell Biol. 2008 Sep;Chapter 1:Unit 1F.5. doi: 10.1002/9780470151808.sc01f05s6.
Abstract
Vascular progenitor cells derived from stem cells could potentially lead to a variety of clinically relevant applications, including cell-based therapies and tissue engineering. Here, we describe methods for isolating purified proliferating populations of vascular endothelial cells from mouse embryonic stem cells (mESC) using Flk-1 positive sorted cells, VEGF supplementation, and a rigorous manual selection technique required for endothelial cell purification and expansion. Using this in vitro derivation procedure, it is possible to obtain millions of cells at various stages of differentiation, with the potential for up to 25 population doublings.
摘要
源自干细胞的血管祖细胞可能会带来多种临床相关应用，包括基于细胞的疗法和组织工程。在此，我们描述了从小鼠胚胎干细胞（mESC）中分离纯化增殖性血管内皮细胞群体的方法，该方法使用Flk-1阳性分选细胞、补充血管内皮生长因子（VEGF），以及内皮细胞纯化和扩增所需的严格手动筛选技术。使用这种体外衍生程序，可以获得数百万处于不同分化阶段的细胞，其潜在的群体倍增次数可达25次。
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本文引用的文献
1
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Circ Res. 2007 Aug 3;101(3):286-94. doi: 10.1161/CIRCRESAHA.107.150201. Epub 2007 Jun 14.
2
Embryonic stem-cell culture as a tool for developmental cell biology.胚胎干细胞培养作为发育细胞生物学的一种工具。
Nat Rev Mol Cell Biol. 2007 Jun;8(6):502-7. doi: 10.1038/nrm2189.
3
Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo.源自人类胚胎干细胞的内皮细胞在体内形成持久的血管。
Nat Biotechnol. 2007 Mar;25(3):317-8. doi: 10.1038/nbt1287. Epub 2007 Feb 25.
4
Differentiation of arterial, venous, and lymphatic endothelial cells from vascular progenitors.从血管祖细胞分化出动脉、静脉和淋巴管内皮细胞。
Trends Cardiovasc Med. 2007 Feb;17(2):59-63. doi: 10.1016/j.tcm.2007.01.001.
5
In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells.人类多能成体祖细胞的体外和体内动脉分化
Blood. 2007 Mar 15;109(6):2634-42. doi: 10.1182/blood-2006-06-030411. Epub 2006 Nov 7.
6
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J Transl Med. 2006 May 8;4:20. doi: 10.1186/1479-5876-4-20.
7
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Hypertension. 2005 Jul;46(1):7-18. doi: 10.1161/01.HYP.0000168923.92885.f7. Epub 2005 Jun 13.
8
Use of embryonic stem cell-derived endothelial cells as a cell source to generate vessel structures in vitro.使用胚胎干细胞衍生的内皮细胞作为细胞来源在体外生成血管结构。
Tissue Eng. 2005 Mar-Apr;11(3-4):497-505. doi: 10.1089/ten.2005.11.497.
9
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Development. 2005 Mar;132(5):873-84. doi: 10.1242/dev.01657. Epub 2005 Jan 26.
10
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Endothelium. 2003;10(6):329-36. doi: 10.1080/10623320390272325.
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