Yang Jaemoon, Eom Kilho, Lim Eun-Kyung, Park Jinsung, Kang Yoonah, Yoon Dae Sung, Na Sungsoo, Koh Eui Kwan, Suh Jin-Suck, Huh Yong-Min, Kwon Tae Yun, Haam Seungjoo
Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea.
Langmuir. 2008 Nov 4;24(21):12112-5. doi: 10.1021/la802184m. Epub 2008 Oct 1.
We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.
我们制备了基于金纳米颗粒（AuNP）的高性能探针用于活癌细胞检测。通过金盐还原法合成了尺寸分布窄（约10纳米）的AuNP，并将其作为交联剂和热点沉积在胺化基底上。在此，AuNP使得抗体（西妥昔单抗）能够轻松高效地固定，该抗体可与表皮癌表面的表皮生长因子受体（EGFR）选择性相互作用，作为检测部分在无需纳米光刻工艺的情况下固定在沉积有AuNP的基底上。西妥昔单抗与沉积有AuNP的基底结合后，作为活癌细胞检测器（LCCD）的西妥昔单抗共轭探针能够检测与上皮癌相关的EGFR高表达A431细胞，与EGFR缺陷的MCF7细胞相比，其特异性和灵敏度高54倍。这意味着基于AuNP的探针在检测和分离小生物分子、细胞及其他化学物质方面显示出巨大潜力。
