Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Anal Chem. 2009 Aug 15;81(16):6641-8. doi: 10.1021/ac900628y.
Gelatin-stabilized gold nanoparticles (AuNPs-gelatin) with hydrophilic and biocompatible were prepared with a simple and "green" route by reducing in situ tetrachloroauric acid in gelatin. The nanoparticles showed the excellent colloidal stability. UV-vis spectra, transmission electron microscopy (TEM), and atomic force microscopy revealed the formation of well-dispersed AuNPs with different sizes. By combination of the biocompatibility of AuNPs and excellent conductivity of carboxylic single-walled carbon nanotubes (c-SWNTs), a novel nanocomposite was designed for the immobilization and cytosensing of HL-60 cells at electrodes. The immobilized cells showed sensitive voltammetric response, good activity, and increased electron-transfer resistance. It can be used as a highly sensitive impedance sensor for HL-60 cells ranging from 1 x 10(4) to 1 x 10(7) cell mL(-1) with a limit of detection of 5 x 10(3) cell mL(-1). Moreover, the nanocomposite could effectively facilitate the interaction of adriamycin (ADR) with HL-60 cells and remarkably enhance the permeation and drug uptake of anticancer agents in the cancer cells, which could readily lead to the induction of the cell death of leukemia cells.
明胶稳定的金纳米粒子（AuNPs-明胶）具有亲水性和生物相容性，通过在明胶中原位还原四氯金酸，采用简单且“绿色”的方法制备。纳米粒子表现出优异的胶体稳定性。紫外可见光谱、透射电子显微镜（TEM）和原子力显微镜揭示了具有不同尺寸的良好分散的 AuNPs 的形成。通过 AuNPs 的生物相容性和羧基单壁碳纳米管（c-SWNTs）的优异导电性的结合，设计了一种新型纳米复合材料，用于在电极上固定和细胞检测 HL-60 细胞。固定化细胞表现出灵敏的伏安响应、良好的活性和增加的电子转移电阻。它可以用作 HL-60 细胞的高灵敏度阻抗传感器，范围从 1 x 10(4)到 1 x 10(7)细胞 mL(-1)，检测限为 5 x 10(3)细胞 mL(-1)。此外，该纳米复合材料可以有效地促进阿霉素（ADR）与 HL-60 细胞的相互作用，并显著增强抗癌药物在癌细胞中的渗透和摄取，这可以很容易地导致白血病细胞的死亡诱导。
