Melo Marcélia Garcez Dória, Araújo Adriano Antunes Souza, Rocha Carla Paula Leite, Almeida Emyle Mayra Santana Alves, Siqueira Rosana de Souza, Bonjardim Leonardo Rigoldi, Quintans Lucindo José
Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), São Cristóvão-SE, CEP 49000-000, Brazil.
Biol Pharm Bull. 2008 Oct;31(10):1977-80. doi: 10.1248/bpb.31.1977.
Lichens and their secondary metabolites have attracted the interest of many researchers. Some species have been shown to contain substances with remarkable biologic activity, as antimicrobial, mainly against Gram positive bacteria; antineoplasic acting on solid and ascetic tumors, or in culture cells; antiviral; hypotensive; and spasmolytic effects. The aim of this study was to isolate and characterize atranorin, one of the major constituents which presents in Cladina kalbii (DES ABB.) AHTI., and analyze its antinociceptive effect. The antinociceptive activity was verified in acetic acid-induced writhing test and formalin test with mice. In this work it was observed that atranorin was effective in significant reducing (p<0.05) abdominal writhing at doses of 200 and 400 mg/kg (p.o.) by 52.6 and 61.3%, respectively, when compared to control group (vehicle). The formalin test showed in 200 and 400 mg/kg (p.o.) that atranorin injection was able to inhibit the inflammatory processes (second phase) dose dependently.
地衣及其次生代谢产物吸引了许多研究人员的关注。一些物种已被证明含有具有显著生物活性的物质，如抗菌活性，主要针对革兰氏阳性菌；对实体瘤和腹水瘤或培养细胞具有抗肿瘤作用；抗病毒；降血压；以及解痉作用。本研究的目的是分离和鉴定存在于卡尔比枝状地衣（DES ABB.）AHTI.中的主要成分之一——阿特拉诺林，并分析其镇痛作用。通过乙酸诱导的小鼠扭体试验和福尔马林试验验证了其镇痛活性。在这项研究中观察到，与对照组（赋形剂）相比，阿特拉诺林在200和400mg/kg（口服）剂量下能有效显著减少（p<0.05）腹部扭体次数，分别减少52.6%和61.3%。福尔马林试验表明，在200和400mg/kg（口服）剂量下，注射阿特拉诺林能够剂量依赖性地抑制炎症过程（第二阶段）。
