Rousset B, Poncet C, Mornex R
Ann Endocrinol (Paris). 1976 Mar-Apr;37(2):109-10.
We have compared the effects of TSH and dibutyryl-cAMP (DBC) on three parameters of iodine metabolism in isolated hog thyroid cells: iodide uptake, thyroxine (T4) synthesis and T4 secretion. As WILSON, we observed a similarity between TSH (60 mU/ml) and DBC (3 mM) actions on iodide uptake and T4 synthesis. But DBC did not reproduce the TSH stimulation of T4 secretion. Since TSH increased T4 secretion in the presence of DBC, the lack of effect of DBC was not due to an alteration of the secretory process by DBC. The present observation might suggest that the stimulatory effect of TSH on T4 secretion by isolated thyroid cells is not mediated by the adenyl cyclase-cAMP system, or could indicate that DBC may not act like cAMP on some target enzyme systems.
我们比较了促甲状腺激素（TSH）和二丁酰环磷腺苷（DBC）对分离的猪甲状腺细胞碘代谢三个参数的影响：碘摄取、甲状腺素（T4）合成和T4分泌。正如威尔逊所观察到的，我们发现TSH（60 mU/ml）和DBC（3 mM）对碘摄取和T4合成的作用相似。但DBC并未重现TSH对T4分泌的刺激作用。由于在存在DBC的情况下TSH增加了T4分泌，DBC缺乏作用并非由于其对分泌过程的改变。目前的观察结果可能表明，TSH对分离的甲状腺细胞T4分泌的刺激作用不是由腺苷酸环化酶 - cAMP系统介导的，或者可能表明DBC在某些靶酶系统上的作用可能与cAMP不同。
