Hua Ngoc-Phuc, Hamza-Chaffai Amel, Vreeland Russell H, Isoda Hiroko, Naganuma Takeshi
Graduate School of Biosphere Science, Hiroshima University, Kagamiyama, Higashi-hiroshima 739-8528, Japan.
Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2409-14. doi: 10.1099/ijs.0.65693-0.
A Gram-positive, endospore-forming, rod-shaped and moderately halophilic bacterium was isolated from a salt-crust sample collected from Gharsa salt lake (Chott el Gharsa), Tunisia. The newly isolated bacterium, designated SA-Vb1(T), was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Strain SA-Vb1(T) was closely related to the type strains of Virgibacillus marismortui and Virgibacillus olivae, with 16S rRNA gene sequence similarities of 99.7 and 99.4 %, respectively. However, strain SA-Vb1(T) was distinguished from these two type strains on the basis of phenotypic characteristics and DNA-DNA relatedness (29.4 and 5.1 %, respectively). The genetic relationship between strain SA-Vb1(T) and Virgibacillus pantothenticus IAM 11061(T) (the type strain of the type species) and other type strains of the genus was 96-98 % based on 16S rRNA gene sequence similarity and 18.3-22.3 % based on DNA-DNA hybridization. Biochemical analysis resulted in determination of major fatty acids iso-C(15 : 0), anteiso-C(15 : 0) and anteiso-C(17 : 0) (33.3, 29.2 and 9.8 %, respectively); phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine were the main polar lipids and MK-7 was the predominant menaquinone ( approximately 100 %). The distinct characteristics demonstrated by strain SA-Vb1(T) represent properties of a novel species of the genus Virgibacillus, for which the name Virgibacillus salarius sp. nov. is proposed. The type strain is SA-Vb1(T) (=JCM 12946(T) =DSM 18441(T)).
从突尼斯加尔萨盐湖（绍特艾尔加尔萨）采集的盐壳样本中分离出一株革兰氏阳性、形成芽孢、杆状且嗜盐适度的细菌。新分离出的细菌命名为SA-Vb1(T)，基于多相分类法进行鉴定，包括基因型、表型和化学分类特征。菌株SA-Vb1(T)与死海芽孢杆菌（Virgibacillus marismortui）和橄榄芽孢杆菌（Virgibacillus olivae）的模式菌株密切相关，16S rRNA基因序列相似性分别为99.7%和99.4%。然而，菌株SA-Vb1(T)在表型特征和DNA-DNA相关性方面（分别为29.4%和5.1%）与这两个模式菌株不同。基于16S rRNA基因序列相似性，菌株SA-Vb1(T)与泛酸芽孢杆菌（Virgibacillus pantothenticus）IAM 11061(T)（模式种的模式菌株）及该属其他模式菌株的遗传关系为96 - 98%，基于DNA-DNA杂交则为18.3 - 22.3%。生化分析确定主要脂肪酸为异-C(15 : 0)、anteiso-C(15 : 0)和anteiso-C(17 : 0)（分别为33.3%、29.2%和9.8%）；磷脂酰甘油、双磷脂酰甘油和磷脂酰乙醇胺是主要极性脂质，MK-7是主要的甲基萘醌（约100%）。菌株SA-Vb1(T)表现出的独特特征代表了芽孢杆菌属一个新物种的特性，为此提出新名称盐湖芽孢杆菌（Virgibacillus salarius sp. nov.）。模式菌株为SA-Vb1(T)（=JCM 12946(T) =DSM 18441(T)）。
