Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
J Microbiol. 2009 Dec;47(6):705-9. doi: 10.1007/s12275-009-0107-9. Epub 2010 Feb 4.
A strictly aerobic Gram-positive, moderately halophilic spore forming bacterium, designated strain SL6-1(T), was isolated from a salt lake in Xin-jiang province, China. Growth of strain SL6-1(T) was observed at NaCl concentrations of 0 approximately 20% (w/v) (the optimum being 5 approximately 7%, w/v). The peptidoglycan type of strain SL6-1(T) was Algamma-meso-diaminopimelic acid and its major cellular fatty acids were iso-C(14:0) and iso-C(16:0) and ante-iso-C(15:0). The major respiratory isoprenoid quinone was MK-7 and the G+C content of the genomic DNA was 44.5 mol%. The major cellular phospholipids were phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SL6-1(T) formed a phylogenetic lineage within the genus Virgibacillus. Based on 16S rRNA gene sequence similarity, the strain was most closely related to Virgibacillus olivae E(30)8(T), Virgibacillus kekensis YIM kkny16(T), Virgibacillus marismortui DSM 12325(T) with 97.1%, 97.1%, and 97.0% gene sequence similarities, respectively and the sequence similarities to other related taxa were less than 96.7%. The DNA relatedness values between strain SL6-1(T) and V. olivae E(30)8(T), V. kekensis YIM kkny16(T), V. marismortui DSM 12325(T) were 16.7%, 51.0%, and 22.8%, respectively. On the basis of physiological, biochemical and phylogenetic properties, strain SL6-1(T) represents a novel species, for which the name Virgibacillus xinjiangensis sp. nov. is proposed. The type strain is SL6-1(T) (=KCTC 13128(T) =DSM 19031(T)).
一株严格需氧、革兰氏阳性、中嗜盐、产芽孢的细菌，被命名为 SL6-1(T) 菌株，从中国新疆的一个盐湖中分离得到。该菌株在 NaCl 浓度为 0 至 20%（w/v）（最佳为 5 至 7%，w/v）的条件下生长。菌株 SL6-1(T) 的肽聚糖类型为 Algamma-meso-diaminopimelic 酸，其主要细胞脂肪酸为 iso-C(14:0) 和 iso-C(16:0) 和 ante-iso-C(15:0)。主要呼吸异戊二烯醌为 MK-7，基因组 DNA 的 G+C 含量为 44.5 mol%。主要细胞磷脂为磷脂酰甘油和双磷脂酰甘油。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SL6-1(T) 在 Virgibacillus 属内形成一个系统发育分支。根据 16S rRNA 基因序列相似性，该菌株与 Virgibacillus olivae E(30)8(T)、Virgibacillus kekensis YIM kkny16(T)、Virgibacillus marismortui DSM 12325(T) 的相似度最高，分别为 97.1%、97.1%和 97.0%，与其他相关分类群的序列相似度均低于 96.7%。菌株 SL6-1(T) 与 V. olivae E(30)8(T)、V. kekensis YIM kkny16(T)、V. marismortui DSM 12325(T) 的 DNA 相关值分别为 16.7%、51.0%和 22.8%。基于生理、生化和系统发育特性，菌株 SL6-1(T) 代表一个新种，命名为新疆盐单胞菌（Virgibacillus xinjiangensis）。其模式菌株为 SL6-1(T)（=KCTC 13128(T) =DSM 19031(T)）。
