Wu X, Yang Y, Han Y, Zhang J, Liang Y, Li H, Li B, Wang L
The Institute for Tuberculosis Research, the Second Affiliated Hospital of Chinese PLA General Hospital, Beijing, China.
J Appl Microbiol. 2008 Oct;105(4):1121-7. doi: 10.1111/j.1365-2672.2008.03850.x.
To determine whether resuscitation-promoting factor (RPF) from Mycobacterium tuberculosis can promote mycobacterial growth and shorten culture time.
We cloned, expressed and purified an RPF from M. tuberculosis, Rv1009 protein and subsequently studied the biological activity of the recombinant Rv1009 (rRv1009) in liquid and on solid media. Our results indicate that the molecular weight of rRv1009 protein expressed in Escherichia coli BL21 was approximately 39 kDa. At picomolar and micromolar concentrations, rRv1009 protein could increase the optical density of freeze-dried Mycobacterium bovis BCG three to fivefold in Middlebrook 7H9 medium, stimulate the growth of viable mycobacteria on solid medium, and shorten positive growth detection time of a small number of M. tuberculosis in BACTEC 960 medium.
The rRv1009 could promote proliferation of mycobacteria. It may be useful for culture of mycobacteria presented in clinical samples.
rRv1009 protein can be used as a growth-promoting reagent of mycobacteria in the medium to shorten the time of culture.
确定结核分枝杆菌的复苏促进因子（RPF）是否能促进分枝杆菌生长并缩短培养时间。
我们克隆、表达并纯化了结核分枝杆菌的一种RPF，即Rv1009蛋白，随后研究了重组Rv1009（rRv1009）在液体和固体培养基中的生物学活性。我们的结果表明，在大肠杆菌BL21中表达的rRv1009蛋白的分子量约为39 kDa。在皮摩尔和微摩尔浓度下，rRv1009蛋白可使冻干的牛分枝杆菌卡介苗在Middlebrook 7H9培养基中的光密度增加三到五倍，刺激固体培养基上活分枝杆菌的生长，并缩短BACTEC 960培养基中少量结核分枝杆菌的阳性生长检测时间。
rRv1009可促进分枝杆菌增殖。它可能对临床样本中分枝杆菌的培养有用。
rRv1009蛋白可作为培养基中分枝杆菌的生长促进试剂，以缩短培养时间。
