Pan Jin-Shui, He Shao-Zhen, Xu Hong-Zhi, Zhan Xiao-Juan, Yang Xiao-Ning, Xiao Hong-Min, Shi Hua-Xiu, Ren Jian-Lin
Zhongshan Hospital, Xiamen University, China.
World J Gastroenterol. 2008 Oct 14;14(38):5857-67. doi: 10.3748/wjg.14.5857.
To study the role of mitochondrial energy disorder in the pathogenesis of ethanol-induced gastric mucosa injury.
Wistar rats were used in this study. A gastric mucosal injury model was established by giving the rats alcohol. Gross and microscopic appearance of gastric mucosa and ultrastructure of mitochondria were evaluated. Malondiadehyde (MDA) in gastric mucosa was measured with thiobarbituric acid. Expression of ATP synthase (ATPase) subunits 6 and 8 in mitochondrial DNA (mtDNA) was determined by reverse transcription polymerase chain reaction (RT-PCR).
The gastric mucosal lesion index was correlated with the MDA content in gastric mucosa. As the concentration of ethanol was elevated and the exposure time to ethanol was extended, the content of MDA in gastric mucosa increased and the extent of damage aggravated. The ultrastructure of mitochondria was positively related to the ethanol concentration and exposure time. The expression of mtDNA ATPase subunits 6 and 8 mRNA declined with the increasing MDA content in gastric mucosa after gavage with ethanol.
Ethanol-induced gastric mucosa injury is related to oxidative stress, which disturbs energy metabolism of mitochondria and plays a critical role in the pathogenesis of ethanol-induced gastric mucosa injury.
研究线粒体能量紊乱在乙醇诱导的胃黏膜损伤发病机制中的作用。
本研究采用Wistar大鼠。通过给大鼠灌胃酒精建立胃黏膜损伤模型。评估胃黏膜的大体和微观外观以及线粒体的超微结构。用硫代巴比妥酸测定胃黏膜中的丙二醛（MDA）。通过逆转录聚合酶链反应（RT-PCR）测定线粒体DNA（mtDNA）中ATP合酶（ATPase）亚基6和8的表达。
胃黏膜损伤指数与胃黏膜中MDA含量相关。随着乙醇浓度升高和乙醇暴露时间延长，胃黏膜中MDA含量增加，损伤程度加重。线粒体超微结构与乙醇浓度和暴露时间呈正相关。乙醇灌胃后，胃黏膜中MDA含量增加，mtDNA ATPase亚基6和8 mRNA的表达下降。
乙醇诱导的胃黏膜损伤与氧化应激有关，氧化应激扰乱线粒体能量代谢，在乙醇诱导的胃黏膜损伤发病机制中起关键作用。
