Nedenskov-Sørensen P, Aase S, Bjørneklett A, Fausa O, Bukholm G
Institute Group of Laboratory Medicine, National Hospital, University of Oslo, Norway.
J Clin Microbiol. 1991 Apr;29(4):672-5. doi: 10.1128/jcm.29.4.672-675.1991.
The methods and sampling procedures used in the diagnosis of Helicobacter pylori infection and chronic active gastritis were evaluated. Five biopsy specimens for bacteriological cultivation and three specimens for histological examination were obtained endoscopically from a defined area of the gastric antral mucosae of 83 patients. An increase in the number of biopsy specimens for cultivation from one to five revealed only one more H. pylori-infected patient. H. pylori was isolated from 31 of 83 patients. Three technically adequate samples for histological examination were obtained from each of 74 patients. Of these 74 patients, chronic active gastritis was diagnosed by demonstration of typical histological changes in all three specimens from each of 20 patients, in two of three specimens from each of 3 patients, and in one of three specimens from 1 patient. The results indicate that one biopsy specimen is sufficient for the isolation of H. pylori, whereas several specimens may be necessary for the histological diagnosis. Chronic active gastritis was found in four patients not infected with H. pylori; on the other hand, H. pylori was isolated from nine patients who showed no signs of chronic active gastritis in any of three samples.
对用于诊断幽门螺杆菌感染和慢性活动性胃炎的方法及采样程序进行了评估。从83例患者胃窦黏膜的特定区域通过内镜获取5份用于细菌培养的活检标本和3份用于组织学检查的标本。将用于培养的活检标本数量从1份增加到5份仅多发现了1例幽门螺杆菌感染患者。83例患者中有31例分离出幽门螺杆菌。从74例患者中每人获取3份技术上合格的用于组织学检查的样本。在这74例患者中，20例患者的所有3份标本、3例患者的3份标本中的2份以及1例患者的3份标本中的1份出现典型组织学变化，从而诊断为慢性活动性胃炎。结果表明，1份活检标本足以分离出幽门螺杆菌，而组织学诊断可能需要几份标本。在4例未感染幽门螺杆菌的患者中发现了慢性活动性胃炎；另一方面，从9例在3份样本中均无慢性活动性胃炎迹象的患者中分离出了幽门螺杆菌。
