Chen T Minsue, Castaneda Manuela, Wanitphakdeedecha Rungsima, Nguyen Tri H, Tarrand Jeffrey J, Soares Mario K
Department of Dermatology, University of Texas, MD Anderson Cancer Center, Houston, TX, USA.
Am J Infect Control. 2009 Apr;37(3):244-6. doi: 10.1016/j.ajic.2008.06.005. Epub 2008 Oct 14.
Surgical site infections have been caused by gentian violet (GV) marking solutions that were contaminated with Mycobaterium chelonae. GV solution is also used in surgery to mark surgical sites. It is commercially available as a solution that may not have been prepared under sterile conditions.
Our objective is to describe a skin marking method that is sterile, effective, and economical.
GV solution; microcentrifuge tubes; and round, wood toothpicks are used as an alternative to the standard surgical marker. GV (4 drops) is dispensed into a microcentrifuge tube. After capping, the tube is autoclaved. The toothpick is used as the writing instrument and dipped into the GV as needed for intraoperative skin marking. Unlike commercially available skin markers, skin moisture will not cause the writing implement (toothpick) to become ineffective; merely dry the skin before skin marking.
Autoclaving the commercially available shelved GV solution ensures sterility. The cost of the GV, toothpicks, and microcentrifuge tubes is approximately $0.10 per operation. In contrast, commercially available surgical markers range in cost from $0.79 to $3.89 per pen (manufactured suggested retail price), a 8- to 39- fold difference.
Infectious precautions should be taken with surgical site marking. Marking solutions should be prepared under sterile conditions in a pharmacy. Alternatively, commercially available nonsterile solutions can be autoclaved to ensure sterility.
手术部位感染是由被龟分枝杆菌污染的龙胆紫(GV)标记溶液引起的。GV溶液也用于手术中标记手术部位。它作为一种可能未在无菌条件下配制的溶液在市场上销售。
我们的目的是描述一种无菌、有效且经济的皮肤标记方法。
使用GV溶液、微量离心管和圆形木质牙签作为标准手术标记物的替代品。将4滴GV溶液滴入微量离心管中。盖上盖子后,将管子进行高压灭菌。牙签用作书写工具,根据术中皮肤标记的需要蘸取GV溶液。与市售皮肤标记物不同,皮肤湿度不会导致书写工具(牙签)失效;只需在皮肤标记前擦干皮肤即可。
对市售货架上的GV溶液进行高压灭菌可确保无菌。GV、牙签和微量离心管的成本约为每次手术0.10美元。相比之下,市售手术标记笔的成本为每支0.79美元至3.89美元(制造商建议零售价),相差8至39倍。
手术部位标记应采取感染预防措施。标记溶液应在药房无菌条件下配制。或者,市售的非无菌溶液可进行高压灭菌以确保无菌。