Richards Julia L, Tang XinJing, Turetsky Anna, Dmochowski Ivan J
Department of Chemistry, University of Pennsylvania, 231 South 34th St., Philadelphia, PA 19104-6323, USA.
Bioorg Med Chem Lett. 2008 Dec 1;18(23):6255-8. doi: 10.1016/j.bmcl.2008.09.093. Epub 2008 Sep 30.
'RNA bandages' are composed of two 6-12-mer 2'-OMe RNA strands complementary to a mRNA target that are joined by a photocleavable linker. These tandem oligonucleotides typically exhibit much higher affinity for the mRNA than the individual strands. An RNA bandage with binding arms of different lengths and a 4-base gap blocked translation in vitro of GFP mRNA; subsequent near-UV irradiation restored translation. This provides a general method of photomodulating hybridization for a variety of oligonucleotide-based technologies.
“RNA绷带”由两条与mRNA靶标互补的6 - 12聚体2'-O-甲基化RNA链组成，它们通过一个可光裂解的连接子连接。这些串联寡核苷酸对mRNA的亲和力通常比单链高得多。一种具有不同长度结合臂和4个碱基间隙的RNA绷带在体外阻断了绿色荧光蛋白（GFP）mRNA的翻译；随后的近紫外照射恢复了翻译。这为各种基于寡核苷酸的技术提供了一种光调节杂交的通用方法。
