Lee J H, Park J W, Lee H B
Polymer Research Laboratory 3, Korea Research Institute of Chemical Technology, Daedeog Danji, Dae Jeon.
Biomaterials. 1991 Jul;12(5):443-8. doi: 10.1016/0142-9612(91)90140-6.
Various polymer surfaces--polyethylene, polypropylene, polystyrene, polyethylene terephthalate and poly(methyl methacrylate)--were modified by water vapour plasma discharge treatment. The plasma-treated polymer surfaces were characterized by water contact angle measurement and electron spectroscopy for chemical analysis. It was observed that by the water vapour plasma treatment, the wettability of the polymer surfaces increases largely and almost all functional groups produced on the surfaces are hydroxyl groups. The interactions of Chinese hamster ovary cells with the polymer surfaces having hydroxyl groups by plasma treatment were investigated. The cells adhered and grown on the polymer surfaces were counted using a haemocytometer and photographed with an inverted microscope with a camera attached. The water vapour plasma-treated polymers showed good adhesion, spreading and growth properties on the surfaces with high hydroxyl group density. We confirmed that the water vapour plasma treatment is a simple and effective method to produce hydroxyl groups on the polymer surfaces, which probably have a positive effect on cell adherence.
通过水蒸气等离子体放电处理对各种聚合物表面——聚乙烯、聚丙烯、聚苯乙烯、聚对苯二甲酸乙二酯和聚甲基丙烯酸甲酯进行了改性。通过测量水接触角和进行化学分析电子能谱对经等离子体处理的聚合物表面进行了表征。观察到，通过水蒸气等离子体处理，聚合物表面的润湿性大幅增加，并且表面产生的几乎所有官能团都是羟基。研究了中国仓鼠卵巢细胞与经等离子体处理具有羟基的聚合物表面之间的相互作用。使用血细胞计数器对粘附并生长在聚合物表面上的细胞进行计数，并用附有相机的倒置显微镜拍照。经水蒸气等离子体处理的聚合物在具有高羟基密度的表面上表现出良好的粘附、铺展和生长特性。我们证实，水蒸气等离子体处理是在聚合物表面产生羟基的一种简单有效的方法，这可能对细胞粘附具有积极作用。
