Department of Science, Faculty of Science and Technology, Rajamangala University of Technology Suvarnabhumi, Huntra, Phra Nakhon Si Ayutthaya, 13000, Thailand.
Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
Curr Microbiol. 2024 Apr 8;81(5):130. doi: 10.1007/s00284-024-03661-5.
During the course of the isolation of actinobacteria from sweet potato field soils collected from Phra Nakhon Si Ayutthaya province of Thailand, strain TS4A08 was isolated and subjected to a polyphasic taxonomic approach. The 16S rRNA gene sequence analysis of strain TS4A08 revealed that it is closely related to the type strains of Saccharopolyspora aridisoli, and Saccharopolyspora endophytica with 98.7%, and 98.6% similarity, respectively. However, phylogenetic analyses using 16S rRNA gene and genome sequences indicated that strain TS4A08 clustered with Saccharopolyspora flava AS4.1520 (98.2% similarity), well-supported by bootstrap values, and formed distinct line from the two closest strains. The average nucleotide identity (ANI) values and digital DNA-DNA hybridization (dDDH) values between the genome sequences of strain TS4A08 and the closest type strains of S. aridisoli, S. endophytica, and S. flava, were 86.1-93.2% and 33.1-49.6%, respectively, which were less than the threshold for the species delineation. The genome size and the DNA G + C content of strain TS4A08 were 6.6 Mbp and 70.5%, respectively. The strain grew well at 25-37 °C, pH range of 7-9, and NaCl concentration of 0-5% (w/v). Whole-cell hydrolysates contained meso-diaminopimelic acid. The major fatty acids were iso-C, anteiso-C, and iso-C. Strain TS4A08 exhibited phosphatidylcholine in its polar lipid profile, with MK-9(H) being the predominant isoprenologue. The strain exhibits typical chemotaxonomic properties of the genus Saccharopolyspora, including arabinose, galactose, and ribose as whole-cell sugars. Strain TS4A08 represents a novel species within the genus Saccharopolyspora, for which the name Saccharopolyspora ipomoeae sp. nov. is proposed. The type strain is TS4A08 (= TBRC 17271 = NBRC 115967).
在从泰国那空那育府采集的甘薯田土壤中分离放线菌的过程中，分离出了菌株 TS4A08，并对其进行了多相分类学研究。菌株 TS4A08 的 16S rRNA 基因序列分析表明，它与土壤放线菌沙福芽孢杆菌和内寄生沙福芽孢杆菌的模式菌株的相似度分别为 98.7%和 98.6%。然而，基于 16S rRNA 基因和基因组序列的系统发育分析表明，菌株 TS4A08 与沙福黄孢菌 AS4.1520 聚类（相似度为 98.2%），并得到了支持bootstrap 值的支持，与这两个最接近的菌株形成了明显的分支。菌株 TS4A08 与最接近的模式菌株沙福芽孢杆菌、内寄生沙福芽孢杆菌和沙福黄孢菌的基因组平均核苷酸同一性（ANI）值和数字 DNA-DNA 杂交（dDDH）值分别为 86.1-93.2%和 33.1-49.6%，均低于种间区分的阈值。菌株 TS4A08 的基因组大小和 DNA G+C 含量分别为 6.6 Mbp 和 70.5%。该菌株在 25-37°C、pH 值范围为 7-9 和 0-5%（w/v）的 NaCl 浓度下生长良好。全细胞水解产物中含有 meso-二氨基庚二酸。主要脂肪酸为异-C、anteiso-C 和异-C。菌株 TS4A08 的极性脂谱中含有磷脂酰胆碱，主要的异戊烯醇为 MK-9(H)。该菌株表现出典型的沙福芽孢杆菌属的化学分类学特性，包括阿拉伯糖、半乳糖和核糖作为全细胞糖。菌株 TS4A08 代表沙福芽孢杆菌属中的一个新种，命名为甘薯沙福芽孢杆菌 sp. nov.。模式菌株为 TS4A08（=TBRC 17271=NBRC 115967）。
