Qin Sheng, Li Jie, Zhang Yu-Qin, Zhu Wen-Yong, Zhao Guo-Zhen, Xu Li-Hua, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2527-33. doi: 10.1099/ijs.0.010793-0. Epub 2009 Jul 21.
A novel Gram-positive, aerobic, spore-forming, endophytic actinomycete, designated strain YIM 61359(T), was isolated from the roots of Maytenus austroyunnanensis plants collected from tropical rainforest in Xishuangbanna, Yunnan Province, south-west China. The strain formed single or cluster spores with smooth surfaces from substrate mycelia. The strain contained meso-diaminopimelic acid in the cell wall and arabinose, xylose, galactose and glucose in whole-cell hydrolysates. The acyl type of the cell-wall polysaccharides was glycolyl. MK-10(H(6)), MK-10(H(8)) and MK-10(H(4)) were the predominant menaquinones. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and several unknown phospholipids. The major fatty acids were iso-C(15 : 0), anteiso-C(15 : 0), C(17 : 0), anteiso-C(17 : 0) and iso-C(16 : 0). The DNA G+C content of strain YIM 61359(T) was 69.7 mol%. These chemotaxonomic data indicated that the strain belongs to the family Micromonosporaceae. Phylogenetic analysis based on 16S rRNA gene sequences also suggested that strain YIM 61359(T) fell within the family Micromonosporaceae. On the basis of morphological and chemotaxonomic data, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain YIM 61359(T) is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Plantactinospora mayteni gen. nov., sp. nov. is proposed. The type strain of Plantactinospora mayteni is YIM 61359(T) (=CCTCC AA 208022(T)=DSM 45238(T)).
从中国西南部云南省西双版纳热带雨林采集的滇南美登木植物根部分离出一株新型革兰氏阳性、需氧、产孢内生放线菌，命名为菌株YIM 61359(T)。该菌株从基内菌丝体形成表面光滑的单个或簇状孢子。该菌株细胞壁含有内消旋二氨基庚二酸，全细胞水解产物含有阿拉伯糖、木糖、半乳糖和葡萄糖。细胞壁多糖的酰基类型为糖基。MK-10(H(6))、MK-10(H(8))和MK-10(H(4))是主要的甲基萘醌。极性脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰肌醇和几种未知的磷脂。主要脂肪酸为异-C(15 : 0)、anteiso-C(15 : 0)、C(17 : 0)、anteiso-C(17 : 0)和异-C(16 : 0)。菌株YIM 61359(T)的DNA G+C含量为69.7 mol%。这些化学分类数据表明该菌株属于小单孢菌科。基于16S rRNA基因序列的系统发育分析也表明菌株YIM 61359(T)属于小单孢菌科。根据形态学和化学分类数据、系统发育分析以及16S rRNA基因特征核苷酸的特征模式，菌株YIM 61359(T)被认为代表小单孢菌科内一个新属的一个新物种，为此提出新属名植物放线孢菌属（Plantactinospora），新种名滇南美登木植物放线孢菌（Plantactinospora mayteni）。滇南美登木植物放线孢菌的模式菌株为YIM 61359(T)（=CCTCC AA 208022(T)=DSM 45238(T)）。
