巴西绿蜂胶中主要酚酸的抗溃疡活性评估
Evaluation of antiulcer activity of the main phenolic acids found in Brazilian Green Propolis.
作者信息
Barros Muriel Primon de, Lemos Marivane, Maistro Edson Luis, Leite Mateus Freire, Sousa João Paulo Barreto, Bastos Jairo Kennup, Andrade Sérgio Faloni de
机构信息
Núcleo de Ciência e Tecnologia, Area de Ciências Biológicas e da Saúde, Universidade do Oeste de Santa Catarina, Campus de Videira, Videira - SC, 89560-000, SC, Brazil.
出版信息
J Ethnopharmacol. 2008 Dec 8;120(3):372-7. doi: 10.1016/j.jep.2008.09.015. Epub 2008 Sep 24.
AIM OF THE STUDY
In a previous study, our group described the gastric protective effect of the hydroalcoholic extract of Brazilian green propolis. The main compounds found in Brazilian green propolis include phenolic acids, such as: caffeic, ferulic, p-coumaric and cinnamic acids. This study was therefore carried out to evaluate the antiulcerogenic property of the main phenolic acids found in Brazilian Green Propolis.
MATERIAL AND METHODS
The anti-ulcer assays were performed using the following protocols: nonsteroidal-antiinflammatory drug (NSAID)-induced ulcer, ethanol-induced ulcer, and stress-induced ulcer. The effects of the phenolic acids on gastric content volume, pH and total acidity, using the pylorus ligated model, were also evaluated.
RESULTS
It was observed that treatment using doses of 50 and 250 mg/kg of caffeic, ferulic, p-coumaric and cinnamic acids and positive controls (omeprazol or cimetidine) significantly diminished the lesion index, the total area of the lesion and the percentage of lesion in comparison with the negative control groups. In addition, the percentage of ulcer inhibition was significantly higher in the groups treated with the different phenolic acids, cimetidine or omeprazol, in all the protocols used, compared with the negative control groups. In the model to determine gastric secretion, using ligated pylorus, treatment with phenolic acids and cimetidine reduced the volume of gastric juice and total acidity and significantly increased the gastric pH (p<0.05), compared with the control group, with the exception of the group treated with 50mg/kg of p-coumaric acid, in which no significant difference was observed, compared with the control. In relation to the acute toxicity, none sign of toxicity was observed when phenolic acids, used in this study, were administered for rats in dose of 2,000 mg/kg.
CONCLUSIONS
In conclusion, the results of this study show that caffeic, ferulic, p-coumaric and cinnamic acids display antiulcer activity.
研究目的
在之前的一项研究中，我们团队描述了巴西绿蜂胶水醇提取物的胃保护作用。巴西绿蜂胶中的主要化合物包括酚酸，如：咖啡酸、阿魏酸、对香豆酸和肉桂酸。因此，本研究旨在评估巴西绿蜂胶中主要酚酸的抗溃疡特性。
材料与方法
采用以下方案进行抗溃疡试验：非甾体抗炎药（NSAID）诱导的溃疡、乙醇诱导的溃疡和应激诱导的溃疡。还使用幽门结扎模型评估了酚酸对胃内容物体积、pH值和总酸度的影响。
结果
观察到，与阴性对照组相比，使用50和250mg/kg剂量的咖啡酸、阿魏酸、对香豆酸和肉桂酸以及阳性对照（奥美拉唑或西咪替丁）进行治疗，显著降低了损伤指数、损伤总面积和损伤百分比。此外，在所有使用的方案中，与阴性对照组相比，用不同酚酸、西咪替丁或奥美拉唑治疗的组中溃疡抑制百分比显著更高。在使用结扎幽门测定胃分泌的模型中，与对照组相比，用酚酸和西咪替丁治疗减少了胃液体积和总酸度，并显著提高了胃pH值（p<0.05），但用50mg/kg对香豆酸治疗的组除外，与对照组相比未观察到显著差异。关于急性毒性，当以2000mg/kg的剂量给大鼠施用本研究中使用的酚酸时，未观察到任何毒性迹象。
结论
总之，本研究结果表明，咖啡酸、阿魏酸、对香豆酸和肉桂酸具有抗溃疡活性。
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