Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, 11451, Riyad, Saudi Arabia.
Research and Laboratories Sector, National Drug and Cosmetic Control Laboratories (NDCCL), Saudi Food and Drug Authority, Riyadh, Saudi Arabia.
Sci Rep. 2024 Sep 12;14(1):21295. doi: 10.1038/s41598-024-72379-y.
Recently, the growth of consumer demand for functional foods with potential nutritional and health benefits led to rapid growth of analytical tools for profiling of bioactive metabolites and assure quality. Bee propolis is one of the most important bee products owing to its myriad health value. As a gummy exudate produced in beehives after harvesting from different plant species, bee propolis contains bioactive secondary metabolites. The current study aims to profiling the chemical composition of propolis samples from Nigeria using HPLC-UV-ELSD and with the aid of NMR-based analysis for assignment of metabolites classes abundant in Nigerian propolis. Red Nigerian propolis samples were subjected to phytochemical analysis using HPLC-UV-ELSD and NMR. Further chromatographic separation of promising fractions was performed by column chromatography and size exclusion chromatography. Screening of the antitrypanosomal and cytotoxic activities against Trypanosoma brucei and human leukemia cell lines (U937), respectively, was performed. The performance of LC-MS permitted identification of the different components from which 13 compound were identified and allowed combination of fractions to afford 9 fractions from which two isoflavonoids were isolated and identified using 1D and 2D NMR analysis with MS as isosativan and Medicarpin. Red Nigerian propolis crude extract showed the highest inhibitory activity at 6.5 µg/ml compared to moderate activity for the isolated compounds with MIC of 7.6 µg/ml and 12.1 µg/ml for medicarpin and isosativan, respectively. Moreover, the fraction RN-6 from the total extract showed the potent cytotoxic effect with IC = 26.5 µg/ml compared to standard diminazen which showed IC = 29.5 µg/ml.
最近，消费者对具有潜在营养和健康益处的功能性食品的需求增长，促使用于分析生物活性代谢物和保证质量的分析工具迅速发展。蜂胶是最重要的蜂产品之一，因为它具有多种健康价值。蜂胶是从不同植物物种中采集后，在蜂巢中产生的粘性渗出物，含有生物活性的次生代谢物。本研究旨在使用 HPLC-UV-ELSD 对来自尼日利亚的蜂胶样品进行化学成分分析，并借助基于 NMR 的分析来确定尼日利亚蜂胶中丰富的代谢物类别。对红色尼日利亚蜂胶样品进行了 HPLC-UV-ELSD 和 NMR 的植物化学分析。通过柱色谱和排阻色谱进一步对有希望的馏分进行了色谱分离。对分别针对 Trypanosoma brucei 和人白血病细胞系（U937）的抗锥虫和细胞毒性活性进行了筛选。LC-MS 的性能允许从不同成分中识别出 13 种化合物，并允许对分数进行组合，从而从其中分离出 9 种分数，其中两种异黄酮使用 1D 和 2D NMR 分析与 MS 一起被鉴定为异沙定和 Medicarpin。与分离化合物的 MIC 为 7.6 µg/ml 和 12.1 µg/ml 相比，红色尼日利亚蜂胶粗提物在 6.5 µg/ml 时显示出最高的抑制活性，而 Medicarpin 和异沙定。此外，总提取物的 RN-6 馏分表现出很强的细胞毒性作用，IC = 26.5 µg/ml，而标准 diminazen 的 IC = 29.5 µg/ml。
