Lamppa G, Jacks C
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Plant Mol Biol. 1991 Mar;16(3):469-74. doi: 10.1007/BF00023997.
Two linked genes, A1 and A2, coding for nearly identical isoforms of the acyl carrier protein (ACP) were isolated from an Arabidopsis thaliana (columbia) genomic library and sequenced. The amino acids deduced from the nucleotide sequence of the two genes indicate they encode distinct transit peptides, but the mature proteins are the same except for residue 79. Both genes are predicted to contain three introns in similar positions, although they differ in sequence and length. The introns interrupt regions coding for a) the transit peptide, b) the junction of the transit peptide and mature protein, and c) the highly conserved domain surrounding serine 38 to which the phosphopantetheine is attached. Primer extension analysis indicates that at least A1 is active in young plants.
从拟南芥（哥伦比亚）基因组文库中分离并测序了两个连锁基因A1和A2，它们编码几乎相同的酰基载体蛋白（ACP）同工型。从这两个基因的核苷酸序列推导的氨基酸表明它们编码不同的转运肽，但除了第79位残基外，成熟蛋白是相同的。尽管这两个基因在序列和长度上有所不同，但预计它们在相似位置都含有三个内含子。这些内含子中断了编码以下区域的序列：a）转运肽；b）转运肽与成熟蛋白的连接处；c）与丝氨酸38周围高度保守结构域相连的磷酸泛酰巯基乙胺附着位点。引物延伸分析表明至少A1在幼嫩植物中具有活性。
