Capaldi Andrew P, Kaplan Tommy, Liu Ying, Habib Naomi, Regev Aviv, Friedman Nir, O'Shea Erin K
Howard Hughes Medical Institute, Harvard University Faculty of Arts and Sciences Center for Systems Biology, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA.
Nat Genet. 2008 Nov;40(11):1300-6. doi: 10.1038/ng.235. Epub 2008 Oct 19.
Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.
细胞利用信号通路、转录因子和启动子的复杂网络来调节基因表达。为深入了解这些网络的结构和功能，我们分析了单突变和多突变菌株中的基因表达，以构建芽殖酵母中Hog1丝裂原活化蛋白激酶（MAPK）依赖性渗透应激反应的定量模型。我们的模型表明，Hog1和一般应激（Msn2/4）通路在信号传导和启动子水平上相互作用，以整合信息并产生依赖于环境的反应。这项研究为识别和表征信号整合与处理在其他基因调控网络中的作用开辟了一条道路。
