School of Materials Science, Japan Advanced Institute of Science and Technology, Asahidai, Nomi, Ishikawa 923-1211, Japan.
Analyst. 2009 Oct;134(10):1994-8. doi: 10.1039/b909597g. Epub 2009 Aug 7.
We generated an aqueous two-phase laminar flow in a microfluidic chip and used the system to isolate leukocyte and erythrocyte cells from whole blood cells. The microfluidic system reduced the effect of gravity in the aqueous two-phase system (ATPS). Poly(ethylene glycol) (PEG) and dextran (Dex) solutions were used as the two phases, and the independent flow rates of the solutions were both 2 microL/min. When hydrophobic and hydrophilic polystyrene beads were introduced into the microfluidic device, the hydrophilic beads moved to the Dex layer and the hydrophobic beads to the interface between the two phases. In the case of living cells, Jurkat cells and erythrocytes moved more efficiently to the PEG and Dex layers, respectively, than they move in a conventional ATPS. When whole blood cells were inserted into the microfluidic chip, leukocytes could be separated from erythrocytes because erythrocytes moved to the Dex layer while leukocytes remained outside of this layer in the microfluidic system. The reported microfluidic chip for the whole blood cell separation can effectively be integrated into a Micro Total Analysis System designed for cell-based clinical, forensic, and environmental analyses.
我们在微流控芯片中产生了一个水相层流，并利用该系统从全血中分离白细胞和红细胞。微流控系统减少了水相系统（ATPS）中重力的影响。聚乙二醇（PEG）和葡聚糖（Dex）溶液被用作两相，且溶液的独立流速均为 2μL/min。当将疏水性和亲水性聚苯乙烯珠引入微流控装置中时，亲水性珠移动到 Dex 层，疏水性珠移动到两相之间的界面。对于活细胞，Jurkat 细胞和红细胞分别比在传统 ATPS 中更有效地移动到 PEG 和 Dex 层。当全血细胞被插入微流控芯片中时，因为红细胞移动到 Dex 层，而白细胞留在微流控系统中的该层之外，所以白细胞可以从红细胞中分离出来。报道的用于全血细胞分离的微流控芯片可以有效地集成到专为基于细胞的临床、法医和环境分析设计的微全分析系统中。
