Hu Dehong, Tian Zhiyuan, Wu Wuwei, Wan Wei, Li Alexander D Q
Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
J Am Chem Soc. 2008 Nov 19;130(46):15279-81. doi: 10.1021/ja805948u. Epub 2008 Oct 22.
Beyond-diffraction-limit optical imaging of cells will reveal biological mechanisms, cellular structures, and physiological processes in nanometer scale. Harnessing the photoswitching properties of spiropyran fluorophores, we achieved nanoresolution fluorescence imaging using photoactuated unimolecular logical switching attained reconstruction (PULSAR) microscopy. The PULSAR microscope successfully resolved nanostructures and subcellular organelles when the photoswitchable nanoparticles containing spiropyran dyes were used as fluorescent probes.
超越衍射极限的细胞光学成像将揭示纳米尺度下的生物机制、细胞结构和生理过程。利用螺吡喃荧光团的光开关特性，我们通过光驱动单分子逻辑开关实现重建（PULSAR）显微镜技术实现了纳米分辨率荧光成像。当使用含有螺吡喃染料的光开关纳米颗粒作为荧光探针时，PULSAR显微镜成功分辨出纳米结构和亚细胞细胞器。
