Urman B, Gomel V, Jetha N
Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
Fertil Steril. 1991 Sep;56(3):563-7.
The aim of this study was to determine the effectiveness of hyaluronic acid solution in preventing intraperitoneal (IP) adhesions.
The study design was prospective, randomized and blinded and involved 83 rats.
Measured serosal injury was inflicted using a CO2 laser on the right uterine horn of the rat. Animals randomized to groups 1 and 2 received either 0.4% hyaluronic acid or its diluent phosphate-buffered saline (PBS) intraperitoneally before and after the injury. In groups 3 and 4, the same solutions were used only after the injury. Postoperative adhesions were assessed at second-look laparotomy. Histologic assessment of the fresh laser injury was carried out on uteri pretreated with hyaluronic acid, PBS, or nothing.
Pretreatment with hyaluronic acid was associated with a significant reduction in postoperative adhesions and a significantly decreased crater depth.
Hyaluronic acid appears to reduce postoperative IP adhesion formation by coating the serosal surfaces and decreasing the extent of initial tissue injury.
本研究旨在确定透明质酸溶液在预防腹腔内粘连方面的有效性。
本研究设计为前瞻性、随机且盲法研究，涉及83只大鼠。
使用二氧化碳激光对大鼠右侧子宫角造成可测量的浆膜损伤。随机分为1组和2组的动物在损伤前后分别腹腔内注射0.4%透明质酸或其稀释剂磷酸盐缓冲盐水（PBS）。3组和4组仅在损伤后使用相同溶液。在二次剖腹探查时评估术后粘连情况。对用透明质酸、PBS预处理或未预处理的子宫进行新鲜激光损伤的组织学评估。
透明质酸预处理与术后粘连显著减少及创口深度显著降低相关。
透明质酸似乎通过覆盖浆膜表面并减少初始组织损伤程度来减少术后腹腔内粘连的形成。
