Phytopathology. 1999 Mar;89(3):239-46. doi: 10.1094/PHYTO.1999.89.3.239.
ABSTRACT A membrane feeding system and polymerase chain reaction (PCR) were used to track squash leaf curl virus (SLCV) DNA in whole whitefly body extracts and in saliva, honeydew, and hemolymph of its whitefly vector, Bemisia tabaci, and a whitefly nonvector, Trialeurodes vaporariorum. SLCV ingestion was monitored by PCR in whiteflies that were given acquisition access periods (AAPs) ranging from 0.5 to 96 h on virus-infected plants. SLCV detection by PCR in whole body extracts was considered reflective of virus ingestion. As whiteflies were given longer AAPs, the number of whiteflies that ingested SLCV increased. SLCV DNA was detected in honeydew of vector and nonvector whiteflies, indicating that virions, viral DNA, or both passed unimpeded through the digestive system. SLCV DNA was detected in saliva and hemolymph of B. tabaci, but not in these fractions from nonvector whiteflies, despite virus ingestion by both. Although vector and nonvector whiteflies both ingested SLCV, only in the vector, B. tabaci, did virus cross the gut barrier, enter the hemolymph, or pass into the salivary system. These results suggest that digestive epithelia of nonvector whiteflies did not permit SLCV passage from the gut to hemocoel, whereas virus effectively crossed the analogous gut barrier in vector whiteflies.
摘要 采用膜饲养系统和聚合酶链反应（PCR）技术，追踪了在整只粉虱体内提取物以及其粉虱媒介烟粉虱（Bemisia tabaci）和非媒介烟粉虱（Trialeurodes vaporariorum）的唾液、蜜露和血淋巴中感染的秋葵花叶卷曲病毒（SLCV）DNA。通过 PCR 监测粉虱取食情况，粉虱在感染植株上的取食接触期（AAP）为 0.5-96 h。整只粉虱体内提取物的 PCR 检测结果被认为反映了粉虱的取食情况。随着取食接触期的延长，取食 SLCV 的粉虱数量增加。在媒介和非媒介粉虱的蜜露中均检测到 SLCV DNA，表明病毒粒子、病毒 DNA 或两者均畅通无阻地通过了消化系统。SLCV DNA 也在 B. tabaci 的唾液和血淋巴中被检测到，但在非媒介粉虱的这些部分中则未被检测到，尽管两者都取食了病毒。尽管媒介和非媒介粉虱都取食了 SLCV，但只有在媒介烟粉虱 B. tabaci 中，病毒才跨越了肠道屏障，进入血淋巴或进入唾液系统。这些结果表明，非媒介粉虱的消化上皮不允许 SLCV 从肠道到血腔的传播，而病毒有效地穿过了媒介粉虱中类似的肠道屏障。
