Phytopathology. 1998 Aug;88(8):851-5. doi: 10.1094/PHYTO.1998.88.8.851.
ABSTRACT Wheatgrass (Thinopyrum intermedium) possesses a high level of resistance to barley yellow dwarf virus (BYDV) subgroup I and subgroup II strains. A wheat line (P29), in which the 7D chromosome has been substituted with a group 7 chromosome from T. intermedium, was examined for the level of resistance to two subgroup I and two subgroup II BYDV strains. In P29 plants inoculated with the subgroup I PAV strains, the titer of virus in leaf and stem tissue was typically reduced 42 to 52% when compared with the BYDV-susceptible cv. Abe. P29 and 'Abe' had the same content of PAV in roots. These results and the absence of detectable virus in inoculated T. intermedium plants indicate that the complete resistance to subgroup I possessed by the wheatgrass has not been introgressed into P29. In contrast, P29 was completely resistant throughout the plant to the subgroup II strains, NY-RPV and NY-RMV, demonstrating that the complete resistance to subgroup II in T. intermedium was incorporated into P29. Further analysis of this resistance to NY-RPV showed that NY-RPV can replicate in mesophyll protoplasts of P29 and 'Abe', suggesting that this resistance is not operating at the single-cell level. Molecular marker analysis confirmed that the T. intermedium chromosome present in P29 is a different group 7 wheatgrass chromosome than that present in L1, a wheat line with BYDV resistance properties similar to those of P29.
摘要 冰草（中间冰草）对大麦黄矮病毒（BYDV）亚组 I 和亚组 II 株系具有高水平的抗性。一种含有中间冰草 7D 染色体组片段的小麦品系（P29），被检测对两种亚组 I 和两种亚组 II BYDV 株系的抗性水平。在接种亚组 I PAV 株系的 P29 植株中，与 BYDV 敏感品种 Abe 相比，叶片和茎组织中的病毒滴度通常降低 42%至 52%。P29 和 Abe 的根中含有相同含量的 PAV。这些结果以及在接种的中间冰草植株中未检测到病毒表明，冰草对亚组 I 的完全抗性尚未被导入 P29。相比之下，P29 对亚组 II 株系 NY-RPV 和 NY-RMV 在整个植株中均表现出完全抗性，表明中间冰草对亚组 II 的完全抗性已被导入 P29。对 NY-RPV 的进一步分析表明，NY-RPV 可以在 P29 和 Abe 的叶肉原生质体中复制，表明这种抗性不是在单细胞水平上起作用。分子标记分析证实，P29 中存在的中间冰草染色体是与具有与 P29 相似 BYDV 抗性特性的 L1 小麦品系中存在的不同的第 7 组小麦草染色体。
