使用TruGene测序系统对人类免疫缺陷病毒1型整合酶抑制剂进行基因型抗逆转录病毒耐药性检测。
Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.
作者信息
Paar Christian, Palmetshofer Christina, Flieger Karin, Geit Maria, Kaiser Rolf, Stekel Herbert, Berg Jörg
机构信息
Institute of Laboratory Medicine, General Hospital Linz, Linz, Austria.
出版信息
J Clin Microbiol. 2008 Dec;46(12):4087-90. doi: 10.1128/JCM.01246-08. Epub 2008 Oct 22.
Abstract
A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with >or=500 RNA copies/ml for mutation detection and HIV-1 subtyping across a spectrum of HIV-1 subtypes.
摘要
利用自动化TruGene测序系统开发了一种测序检测方法，用于检测对1型人类免疫缺陷病毒（HIV-1）整合酶抑制剂拉替拉韦和埃替拉韦产生耐药性的突变。对于每毫升RNA拷贝数≥500的样本，该检测方法在一系列HIV-1亚型中进行突变检测和HIV-1亚型分型时，均能给出清晰的测序结果。
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本文引用的文献
1
Antiretroviral drug resistance testing in adult HIV-1 infection: 2008 recommendations of an International AIDS Society-USA panel.成人HIV-1感染的抗逆转录病毒药物耐药性检测：美国国际艾滋病协会专家组2008年建议
Clin Infect Dis. 2008 Jul 15;47(2):266-85. doi: 10.1086/589297.
2
Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro.与雷特格韦治疗失败相关的突变在体外会影响整合酶对该抑制剂的敏感性。
Antimicrob Agents Chemother. 2008 Apr;52(4):1351-8. doi: 10.1128/AAC.01228-07. Epub 2008 Jan 28.
3
HIV-1 integrase inhibitors: update and perspectives.HIV-1整合酶抑制剂：最新进展与展望
Adv Pharmacol. 2008;56:199-228. doi: 10.1016/S1054-3589(07)56007-2.
4
Broad antiretroviral activity and resistance profile of the novel human immunodeficiency virus integrase inhibitor elvitegravir (JTK-303/GS-9137).新型人类免疫缺陷病毒整合酶抑制剂埃替格韦（JTK-303/GS-9137）的广泛抗逆转录病毒活性及耐药情况
J Virol. 2008 Jan;82(2):764-74. doi: 10.1128/JVI.01534-07. Epub 2007 Oct 31.
5
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7
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8
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