• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用TruGene测序系统对人类免疫缺陷病毒1型整合酶抑制剂进行基因型抗逆转录病毒耐药性检测。

Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.

作者信息

Paar Christian, Palmetshofer Christina, Flieger Karin, Geit Maria, Kaiser Rolf, Stekel Herbert, Berg Jörg

机构信息

Institute of Laboratory Medicine, General Hospital Linz, Linz, Austria.

出版信息

J Clin Microbiol. 2008 Dec;46(12):4087-90. doi: 10.1128/JCM.01246-08. Epub 2008 Oct 22.

DOI:10.1128/JCM.01246-08
PMID:18945845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2593279/
Abstract

A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with >or=500 RNA copies/ml for mutation detection and HIV-1 subtyping across a spectrum of HIV-1 subtypes.

摘要

利用自动化TruGene测序系统开发了一种测序检测方法，用于检测对1型人类免疫缺陷病毒（HIV-1）整合酶抑制剂拉替拉韦和埃替拉韦产生耐药性的突变。对于每毫升RNA拷贝数≥500的样本，该检测方法在一系列HIV-1亚型中进行突变检测和HIV-1亚型分型时，均能给出清晰的测序结果。

相似文献

1
Genotypic antiretroviral resistance testing for human immunodeficiency virus type 1 integrase inhibitors by use of the TruGene sequencing system.使用TruGene测序系统对人类免疫缺陷病毒1型整合酶抑制剂进行基因型抗逆转录病毒耐药性检测。
J Clin Microbiol. 2008 Dec;46(12):4087-90. doi: 10.1128/JCM.01246-08. Epub 2008 Oct 22.
2
Performance of the TruGene human immunodeficiency virus type 1 genotyping kit and OpenGene DNA sequencing system on clinical samples diluted to approximately 100 copies per milliliter.TruGene 1型人类免疫缺陷病毒基因分型试剂盒及OpenGene DNA测序系统在稀释至每毫升约100拷贝的临床样本上的性能。
Clin Vaccine Immunol. 2006 Feb;13(2):235-8. doi: 10.1128/CVI.13.2.235-238.2006.
3
Human immunodeficiency virus type 1 drug resistance testing: a comparison of three sequence-based methods.1型人类免疫缺陷病毒耐药性检测：三种基于序列的方法的比较
J Clin Microbiol. 2001 Jun;39(6):2157-65. doi: 10.1128/JCM.39.6.2157-2165.2001.
4
Genotypic/phenotypic patterns of HIV-1 integrase resistance to raltegravir.HIV-1 整合酶对拉替拉韦耐药的基因/表型模式。
J Antimicrob Chemother. 2010 Mar;65(3):425-33. doi: 10.1093/jac/dkp477. Epub 2010 Jan 7.
5
Development and optimization of an internally controlled dried blood spot assay for surveillance of human immunodeficiency virus type-1 drug resistance.一种用于监测人类免疫缺陷病毒1型耐药性的内控干血斑检测方法的开发与优化。
J Antimicrob Chemother. 2008 Dec;62(6):1191-8. doi: 10.1093/jac/dkn412. Epub 2008 Oct 16.
6
Comparison of DNA sequencing and a line probe assay for detection of human immunodeficiency virus type 1 drug resistance mutations in patients failing highly active antiretroviral therapy.对接受高效抗逆转录病毒治疗失败的患者，比较DNA测序和线性探针分析检测1型人类免疫缺陷病毒耐药突变的效果。
J Clin Microbiol. 2001 Feb;39(2):454-9. doi: 10.1128/JCM.39.2.454-459.2001.
7
The HIV-1 integrase G118R mutation confers raltegravir resistance to the CRF02_AG HIV-1 subtype.HIV-1 整合酶 G118R 突变使 CRF02_AG 型 HIV-1 对拉替拉韦产生耐药性。
J Antimicrob Chemother. 2011 Dec;66(12):2827-30. doi: 10.1093/jac/dkr389. Epub 2011 Sep 19.
8
Human immunodeficiency virus type 1 drug resistance testing: Evaluation of a new ultra-deep sequencing-based protocol and comparison with the TRUGENE HIV-1 Genotyping Kit.人类免疫缺陷病毒 1 型耐药性检测：一种新的基于超高深度测序的方案评估及其与 TRUGENE HIV-1 基因分型试剂盒的比较。
J Virol Methods. 2011 Dec;178(1-2):94-7. doi: 10.1016/j.jviromet.2011.08.020. Epub 2011 Aug 27.
9
Both human immunodeficiency virus cellular DNA sequencing and plasma RNA sequencing are useful for detection of drug resistance mutations in blood samples from antiretroviral-drug-naive patients.人类免疫缺陷病毒细胞DNA测序和血浆RNA测序对于检测初治抗逆转录病毒药物患者血样中的耐药性突变均有用。
J Clin Microbiol. 2007 Jun;45(6):1783-8. doi: 10.1128/JCM.00056-07. Epub 2007 Apr 18.
10
Evaluation of dried blood spot specimens for HIV-1 drug-resistance testing using the Trugene HIV-1 genotyping assay.使用Trugene HIV-1基因分型检测法评估用于HIV-1耐药性检测的干血斑样本。
J Clin Virol. 2008 Apr;41(4):283-7. doi: 10.1016/j.jcv.2007.12.011. Epub 2008 Feb 20.

引用本文的文献

1
Towards Next-Generation Sequencing for HIV-1 Drug Resistance Testing in a Clinical Setting.迈向临床环境中下一代 HIV-1 耐药性检测的测序技术。
Viruses. 2022 Oct 7;14(10):2208. doi: 10.3390/v14102208.
2
Next-Generation Sequencing to Help Monitor Patients Infected with HIV: Ready for Clinical Use?下一代测序技术能否帮助监测感染 HIV 的患者：是否准备好用于临床？
Curr Infect Dis Rep. 2014 Apr;16(4):401. doi: 10.1007/s11908-014-0401-5.
3
Sensitive deep-sequencing-based HIV-1 genotyping assay to simultaneously determine susceptibility to protease, reverse transcriptase, integrase, and maturation inhibitors, as well as HIV-1 coreceptor tropism.基于深度测序的灵敏HIV-1基因分型检测方法，可同时测定对蛋白酶、逆转录酶、整合酶和成熟抑制剂的敏感性，以及HIV-1共受体嗜性。
Antimicrob Agents Chemother. 2014;58(4):2167-85. doi: 10.1128/AAC.02710-13. Epub 2014 Jan 27.
4
Genotypic prediction of human immunodeficiency virus type 1 tropism by use of plasma and peripheral blood mononuclear cells in the routine clinical laboratory.在常规临床实验室中，通过使用血浆和外周血单核细胞对人类免疫缺陷病毒 1 型的嗜性进行基因预测。
J Clin Microbiol. 2011 Jul;49(7):2697-9. doi: 10.1128/JCM.00336-11. Epub 2011 May 18.
5
Evaluation of two human immunodeficiency virus-1 genotyping systems: ViroSeq 2.0 and an in-house method.两种人类免疫缺陷病毒1型基因分型系统的评估：ViroSeq 2.0和一种内部方法。
J Virol Methods. 2009 Aug;159(2):211-6. doi: 10.1016/j.jviromet.2009.03.021. Epub 2009 Apr 2.

本文引用的文献

1
Antiretroviral drug resistance testing in adult HIV-1 infection: 2008 recommendations of an International AIDS Society-USA panel.成人HIV-1感染的抗逆转录病毒药物耐药性检测：美国国际艾滋病协会专家组2008年建议
Clin Infect Dis. 2008 Jul 15;47(2):266-85. doi: 10.1086/589297.
2
Mutations associated with failure of raltegravir treatment affect integrase sensitivity to the inhibitor in vitro.与雷特格韦治疗失败相关的突变在体外会影响整合酶对该抑制剂的敏感性。
Antimicrob Agents Chemother. 2008 Apr;52(4):1351-8. doi: 10.1128/AAC.01228-07. Epub 2008 Jan 28.
3
HIV-1 integrase inhibitors: update and perspectives.HIV-1整合酶抑制剂：最新进展与展望
Adv Pharmacol. 2008;56:199-228. doi: 10.1016/S1054-3589(07)56007-2.
4
Broad antiretroviral activity and resistance profile of the novel human immunodeficiency virus integrase inhibitor elvitegravir (JTK-303/GS-9137).新型人类免疫缺陷病毒整合酶抑制剂埃替格韦（JTK-303/GS-9137）的广泛抗逆转录病毒活性及耐药情况
J Virol. 2008 Jan;82(2):764-74. doi: 10.1128/JVI.01534-07. Epub 2007 Oct 31.
5
Natural polymorphism of the HIV-1 integrase gene and mutations associated with integrase inhibitor resistance.HIV-1整合酶基因的自然多态性及与整合酶抑制剂耐药相关的突变。
Antivir Ther. 2007;12(4):563-70.
6
Performance of the TruGene human immunodeficiency virus type 1 genotyping kit and OpenGene DNA sequencing system on clinical samples diluted to approximately 100 copies per milliliter.TruGene 1型人类免疫缺陷病毒基因分型试剂盒及OpenGene DNA测序系统在稀释至每毫升约100拷贝的临床样本上的性能。
Clin Vaccine Immunol. 2006 Feb;13(2):235-8. doi: 10.1128/CVI.13.2.235-238.2006.
7
Computational methods for the design of effective therapies against drug resistant HIV strains.针对耐药性HIV毒株设计有效疗法的计算方法。
Bioinformatics. 2005 Nov 1;21(21):3943-50. doi: 10.1093/bioinformatics/bti654. Epub 2005 Sep 6.
8
Integrase inhibitors to treat HIV/AIDS.用于治疗艾滋病毒/艾滋病的整合酶抑制剂。
Nat Rev Drug Discov. 2005 Mar;4(3):236-48. doi: 10.1038/nrd1660.
9
A web-based genotyping resource for viral sequences.一个基于网络的病毒序列基因分型资源。
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W654-9. doi: 10.1093/nar/gkh419.
10
Performance characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA Sequencing System.TRUGENE HIV-1基因分型试剂盒和Opengene DNA测序系统的性能特征。
J Clin Microbiol. 2003 Apr;41(4):1594-9. doi: 10.1128/JCM.41.4.1594-1599.2003.