Wu Zhen, Lin Zhiming, Wei Qiujing, Gu Jieruo
Department of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
Rheumatol Int. 2009 Feb;29(4):389-92. doi: 10.1007/s00296-008-0743-0. Epub 2008 Oct 25.
The objective of this study is to investigate the relationship between clinical features of ankylosing spondylitis (AS) and HLA-B27 status or its subtypes. Clinical data and blood samples were collected with patients' informed consent. Luminex liquid array combining polymerase chain reaction-sequence specific oligonucleotide probe was used to do the low-resolution HLA-B genotype typing. Polymerase chain reaction-sequence specific primer was applied to do the high resolution HLA-B27 typing. In 98 subjects, 93 were HLA-B27 positive, of which three subtypes were detected: B2704 (n = 76), B2705 (n = 12), and B2715 (n = 5). The onset age for B27 negative and positive group was 28 +/- 7.9 and 21.1 +/- 6.2 years, respectively (chi(2) = -2.047, P = 0.041). The onset age for B2704, B2705 and B2715 group was 20.45 +/- 4.50, 26.67 +/- 9.95 and 17.8 +/- 11.12 years, respectively (chi(2) = 7.888, P = 0.019). No significant difference was found between B27 positive and negative group, or among three B27 subtypes groups for other clinical features. In conclusion, the clinical features of AS may be correlated with HLA-B27 status and its polymorphism.
本研究的目的是调查强直性脊柱炎（AS）的临床特征与HLA - B27状态或其亚型之间的关系。在患者知情同意的情况下收集临床数据和血样。采用聚合酶链反应 - 序列特异性寡核苷酸探针结合的Luminex液相芯片进行低分辨率HLA - B基因型分型。应用聚合酶链反应 - 序列特异性引物进行高分辨率HLA - B27分型。在98名受试者中，93名HLA - B27阳性，检测到三种亚型：B2704（n = 76）、B2705（n = 12）和B2715（n = 5）。B27阴性和阳性组的发病年龄分别为28±7.9岁和21.1±6.2岁（χ² = -2.047，P = 0.041）。B2704、B2705和B2715组的发病年龄分别为20.45±4.50岁、26.67±9.95岁和17.8±11.12岁（χ² = 7.888，P = 0.019）。B27阳性和阴性组之间或三种B27亚型组之间的其他临床特征未发现显著差异。总之，AS的临床特征可能与HLA - B27状态及其多态性相关。
