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Evid Based Complement Alternat Med. 2009 Mar;6(1):113-21. doi: 10.1093/ecam/nem071. Epub 2007 Oct 18.
The antioxidant activity of four honey samples from different floral sources (Acacia, Coriander, Sider and Palm) were evaluated with three different assays; DPPH free radical scavenging assay, superoxide anion generated in xanthine-xanthine oxidase (XOD) system and low density lipoprotein (LDL) peroxidation assay. The dark Palm and Sider honeys had the highest antioxidant activity in the DPPH assay. But all the honey samples exhibited more or less the same highly significant antioxidant activity within the concentration of 1mg honey/1 ml in XOD system and LDL peroxidation assays. The chemical composition of these samples was investigated by GC/MS and HPLC analysis, 11 compounds being new to honey. The GC/MS revealed the presence of 90 compounds, mainly aliphatic acids (37 compounds), which represent 54.73, 8.72, 22.87 and 64.10% and phenolic acids (15 compound) 2.3, 1.02, 2.07 and 11.68% for Acacia, Coriander, Sider and Palm honeys. In HPLC analysis, 19 flavonoids were identified. Coriander and Sider honeys were characterized by the presence of large amounts of flavonoids.
四种不同花源(刺槐、芫荽、沙枣和棕榈)的蜂蜜的抗氧化活性分别用三种不同的方法进行了评估:DPPH 自由基清除测定法、黄嘌呤-黄嘌呤氧化酶(XOD)系统中生成的超氧阴离子和低密度脂蛋白(LDL)过氧化测定法。深色棕榈和沙枣蜂蜜在 DPPH 测定法中具有最高的抗氧化活性。但所有的蜂蜜样品在 XOD 系统和 LDL 过氧化测定法中浓度为 1mg 蜂蜜/1ml 时,均表现出或多或少相同的高度显著抗氧化活性。这些样品的化学成分通过 GC/MS 和 HPLC 分析进行了研究,其中 11 种化合物是蜂蜜中的新成分。GC/MS 显示存在 90 种化合物,主要是脂族酸(37 种化合物),分别占 54.73%、8.72%、22.87%和 64.10%和酚酸(15 种化合物)2.3%、1.02%、2.07%和 11.68%。在 HPLC 分析中,鉴定出 19 种类黄酮。芫荽和沙枣蜂蜜的特点是含有大量的类黄酮。