Hwang Hye-Jeong, Lee Hye-Jung, Kim Chang-Ju, Shim Insop, Hahm Dae-Hyun
Acupuncture and Meridian Science Research Center.
J Microbiol Biotechnol. 2008 Oct;18(10):1641-7.
Amygdalin is a cyanogenic glycoside plant compound found in the seeds of rosaceous stone fruits. We evaluated the antiinflammatory and analgesic activities of amygdalin, using an in vitro lipopolysaccharide (LPS)-induced cell line and a rat model with carrageenan-induced ankle arthritis. One mM amygdalin significantly inhibited the expression of TNF-alpha and IL-1beta mRNAs in LPS-treated RAW 264.7 cells. Amygdalin (0.005, 0.05, and 0.1 mg/kg) was intramuscularly injected immediately after the induction of carrageenan-induced arthritic pain in rats, and the anti-arthritic effect of amygdalin was assessed by measuring the weight distribution ratio of the bearing forces of both feet and the ankle circumference, and by analyzing the expression levels of three molecular markers of pain and inflammation (c-Fos, TNF-alpha, and IL-1beta) in the spinal cord. The hyperalgesia of the arthritic ankle was alleviated most significantly by the injection of 0.005 mg/kg amygdalin. At this dosage, the expressions of c-Fos, TNF-alpha, and IL-1beta in the spinal cord were significantly inhibited. However, at dosage greater than 0.005 mg/kg, the painrelieving effect of amygdalin was not observed. Thus, amygdalin treatment effectively alleviated responses to LPStreatment in RAW 264.7 cells and carrageenan-induced arthritis in rats, and may serve as an analgesic for relieving inflammatory pain.
苦杏仁苷是一种在蔷薇科核果种子中发现的含氰糖苷类植物化合物。我们使用体外脂多糖（LPS）诱导的细胞系和角叉菜胶诱导的大鼠踝关节关节炎模型，评估了苦杏仁苷的抗炎和镇痛活性。1 mM苦杏仁苷显著抑制了LPS处理的RAW 264.7细胞中TNF-α和IL-1β mRNA的表达。在大鼠角叉菜胶诱导的关节疼痛发作后，立即肌肉注射苦杏仁苷（0.005、0.05和0.1 mg/kg），通过测量双足承重力量的重量分布比例和踝关节周长，并分析脊髓中疼痛和炎症的三个分子标志物（c-Fos、TNF-α和IL-1β）的表达水平，评估苦杏仁苷的抗关节炎作用。注射0.005 mg/kg苦杏仁苷最显著地减轻了关节炎踝关节的痛觉过敏。在此剂量下，脊髓中c-Fos、TNF-α和IL-1β的表达受到显著抑制。然而，在剂量大于0.005 mg/kg时，未观察到苦杏仁苷的止痛效果。因此，苦杏仁苷治疗有效减轻了RAW 264.7细胞对LPS处理的反应以及大鼠角叉菜胶诱导的关节炎反应，并且可能作为一种缓解炎性疼痛的镇痛药。
