Burnett Elton, Ishida Maria, de Janon Sofia, Naushad Sohail, Duceppe Marc-Olivier, Gao Ruimin, Jardim Armando, Chen Jessica C, Tagg Kaitlin A, Ogunremi Dele, Vinueza-Burgos Christian
Institute of Parasitology, McGill University, Montreal, QC H9X 3V9, Canada.
Division of Food Laboratory, New York State Department of Agriculture and Markets, Albany, NY 12206, USA.
Antibiotics (Basel). 2021 Mar 5;10(3):267. doi: 10.3390/antibiotics10030267.
Infantis, a common contaminant of poultry products, is known to harbor mobile genetic elements that confer multi-drug resistance (MDR) and have been detected in many continents. Here, we report four MDR . strains recovered from poultry house environments in Santa Cruz Island of the Galapagos showing extended-spectrum β-lactamase (ESBL) resistance and reduced fluoroquinolone susceptibility. Whole-genome sequencing (WGS) revealed the presence of the ESBL-conferring gene in an IncFIB-like plasmid in three . Infantis isolates. Multi-locus sequence typing (MLST) and single nucleotide variant/polymorphism (SNP) SNVPhyl analysis showed that the . Infantis isolates belong to sequence type ST32, likely share a common ancestor, and are closely related (1-3 SNP difference) to -containing clinical and veterinary . Infantis isolates from the United States and Latin America. Furthermore, phylogenetic analysis of SNPs following core-genome alignment (i.e., ParSNP) inferred close relatedness between the . Infantis isolates from Galapagos and the United States. Prophage typing confirmed the close relationship among the Galapagos . Infantis and was useful in distinguishing them from the United States isolates. This is the first report of MDR -containing . Infantis in the Galapagos Islands and highlights the need for increased monitoring and surveillance programs to determine prevalence, sources, and reservoirs of MDR pathogens.
婴儿沙门氏菌是家禽产品中常见的污染物，已知其携带可赋予多重耐药性（MDR）的移动遗传元件，并且已在许多大洲被检测到。在此，我们报告了从加拉帕戈斯群岛圣克鲁斯岛的家禽舍环境中分离出的4株多重耐药菌株，这些菌株表现出超广谱β-内酰胺酶（ESBL）耐药性且对氟喹诺酮类药物的敏感性降低。全基因组测序（WGS）显示，在3株婴儿沙门氏菌分离株的一种IncFIB样质粒中存在赋予ESBL的基因。多位点序列分型（MLST）和单核苷酸变异/多态性（SNP）SNVPhyl分析表明，这些婴儿沙门氏菌分离株属于序列型ST32，可能有一个共同的祖先，并且与来自美国和拉丁美洲的含该菌的临床及兽医分离株密切相关（单核苷酸多态性差异为1 - 3个）。此外，基于核心基因组比对的SNP系统发育分析（即ParSNP）推断出加拉帕戈斯群岛的婴儿沙门氏菌分离株与美国的分离株关系密切。原噬菌体分型证实了加拉帕戈斯群岛婴儿沙门氏菌之间的密切关系，并且有助于将它们与美国的分离株区分开来。这是加拉帕戈斯群岛含多重耐药性婴儿沙门氏菌的首次报告，强调了需要加强监测和监督计划，以确定多重耐药病原体的流行情况、来源和宿主。
