Spano Nadia, Casula Lucia, Panzanelli Angelo, Pilo Maria I, Piu Paola C, Scanu Roberta, Tapparo Andrea, Sanna Gavino
Università degli Studi di Sassari, Dipartimento di Chimica, via Vienna 2, 07100 Sassari, Italy.
Talanta. 2006 Feb 15;68(4):1390-5. doi: 10.1016/j.talanta.2005.08.003. Epub 2005 Sep 8.
The use of the RP-HPLC official method of the International Honey Commission (IHC) for the determination of 5-hydroxymethylfurfural (HMF) in strawberry tree honey (Arbutus unedo, a typical Sardinian honey) has brought to light a specific and heavy chromatographic interference that prevents accurate quantification. The interference has been identified as homogentisic acid (HA), i.e. the marker of the botanical origin of the honey. For this reason, an alternative RP-HPLC method is proposed. The bias-free method allows a complete separation of HMF from HA to the baseline level and is faster and more precise than the RP-HPLC official method: the detection and quantification limits are 1.9 and 4.0mgkg(-1), respectively, whereas the repeatability is ca. 2% in the HMF concentration range of 5-140mgkg(-1).
国际蜂蜜委员会（IHC）的RP-HPLC官方方法用于测定草莓树蜂蜜（杨梅，一种典型的撒丁岛蜂蜜）中的5-羟甲基糠醛（HMF），结果发现了一种特定且严重的色谱干扰，这妨碍了准确的定量分析。该干扰物已被鉴定为尿黑酸（HA），即蜂蜜植物来源的标志物。因此，提出了一种替代的RP-HPLC方法。这种无偏差方法能够将HMF与HA完全分离至基线水平，并且比RP-HPLC官方方法更快、更精确：检测限和定量限分别为1.9和4.0mgkg(-1)，而在5-140mgkg(-1)的HMF浓度范围内，重复性约为2%。
