Flisiak Iwona, Zaniewski Przemyslaw, Chodynicka Bozena
Department of Dermatology and Venereology, Medical University of Bialystok, Bialystok, Poland.
Biomarkers. 2008 Aug;13(5):549-56. doi: 10.1080/13547500802033300.
Plasma levels of transforming growth factor (TGF)-beta1, tissue inhibitors of metalloproteinases (TIMP)-1, matrix metalloproteinase (MMP)-1 and interleukin (IL)-18 when analyzed separately demonstrate an association with psoriasis severity and treatment efficacy. To determine the highest correlation with the Psoriasis Area and Severity Index (PASI) score we carried out an analysis of these four proteins combined as the TTMI score. Concentrations of proteins were measured using an enzyme immunoassay in the plasma of 32 patients with chronic plaque-type psoriasis. The concentration of each biomarker was multiplied by the respective coefficient and the final individual TTMI score was the sum of these four values. TGF-beta1, TIMP-1 and IL-18 demonstrated significant positive correlation, whereas MMP-1 demonstrated significant negative correlation with the PASI score. The TTMI score calculated for individual patients varied from -79620 to 145713 (43050+/-8081) and demonstrated significant correlation with the PASI score. The lowest TTMI mean value was observed in patients with a PASI score <16 and the highest value was in patients with a PASI score >20. The combined measurement of plasma TGF-beta1, TIMP-1, MMP-1 and IL-18 has superior value as a biomarker of psoriasis activity in comparison with their separate analysis.
单独分析时，血浆中转化生长因子（TGF）-β1、金属蛋白酶组织抑制剂（TIMP）-1、基质金属蛋白酶（MMP）-1和白细胞介素（IL）-18的水平与银屑病严重程度及治疗效果相关。为确定与银屑病面积和严重程度指数（PASI）评分的最高相关性，我们对这四种蛋白合并为TTMI评分进行了分析。采用酶免疫测定法检测了32例慢性斑块型银屑病患者血浆中这些蛋白的浓度。将每个生物标志物的浓度乘以各自的系数，最终的个体TTMI评分是这四个值的总和。TGF-β1、TIMP-1和IL-18与PASI评分呈显著正相关，而MMP-1与PASI评分呈显著负相关。个体患者计算出的TTMI评分在-79620至145713之间（43050±8081），且与PASI评分呈显著相关。PASI评分<16的患者中观察到最低的TTMI平均值，而PASI评分>20的患者中观察到最高值。与单独分析相比，血浆TGF-β1、TIMP-1、MMP-1和IL-18的联合检测作为银屑病活动生物标志物具有更高的价值。
