Salehpour Mehran, Forsgard Niklas, Possnert Göran
Department of Engineering Sciences, Uppsala University, SE-751 21 Uppsala, Sweden.
Rapid Commun Mass Spectrom. 2008 Dec;22(23):3928-34. doi: 10.1002/rcm.3808.
Accelerator mass spectrometry (AMS) is an ultra-sensitive technique for isotopic ratio measurements. In the biomedical field, AMS can be used to measure femtomolar concentrations of labeled drugs in body fluids, with direct applications in early drug development such as Microdosing. Likewise, the regenerative properties of cells which are of fundamental significance in stem-cell research can be determined with an accuracy of a few years by AMS analysis of human DNA. However, AMS nominally requires about 1 mg of carbon per sample which is not always available when dealing with specific body substances such as localized, organ-specific DNA samples. Consequently, it is of analytical interest to develop methods for the routine analysis of small samples in the range of a few tens of microg. We have used a 5 MV Pelletron tandem accelerator to study small biological samples using AMS. Different methods are presented and compared. A (12)C-carrier sample preparation method is described which is potentially more sensitive and less susceptible to contamination than the standard procedures.
加速器质谱法（AMS）是一种用于同位素比率测量的超灵敏技术。在生物医学领域，AMS可用于测量体液中飞摩尔浓度的标记药物，直接应用于早期药物研发，如微剂量给药。同样，通过对人类DNA进行AMS分析，干细胞研究中具有根本重要性的细胞再生特性能够以几年的精度确定。然而，AMS名义上每个样本需要约1毫克碳，在处理特定身体物质（如局部的、器官特异性的DNA样本）时，这一数量并不总是可得。因此，开发用于几十微克范围内小样本常规分析的方法具有分析意义。我们使用一台5兆伏的佩莱顿串联加速器，利用AMS研究小生物样本。文中介绍并比较了不同方法。描述了一种¹²C载体样本制备方法，该方法可能比标准程序更灵敏且更不易受污染。
