Kahaleh M B
Department of Medicine, Medical University of South Carolina, Charleston 29425.
Clin Immunol Immunopathol. 1991 Jan;58(1):139-44. doi: 10.1016/0090-1229(91)90155-4.
To investigate the role of immune mechanisms in scleroderma (systemic sclerosis, SSc), we measured the levels of selected cytokines and soluble immune markers in patient sera. Forty-two patients and 14 matched healthy controls are the subject of this report. In the SSc group, tumor necrosis factor (TNF) was found in 8/42 (29 +/- 539 pg/ml, mean level +/- SD) and lymphotoxin in 36/42 (1:409-1:200, serum dilution). Interleukin beta (IL-1 beta) was observed in 23/42 (44 +/- 29, U/ml). IL-2 was identified in 36/42 patients with a mean level of 286 +/- 406 U/ml, soluble interleukin-2 receptor in 42/42 (1055 +/- 393, U/ml), soluble CD4 antigen in 27/42 (1:10-1:320, serum dilution), and CD8 in 42/42 (470 +/- 134, U/ml). TNF, lymphotoxin, IL-1 beta, Il-2, and CD4 were not detected in the control group. IL-2 receptor levels in control subjects were 520 +/- 171 U/ml, significantly lower than those of scleroderma (P less than 0.001), and CD8 levels (582 +/- 140) were significantly higher than in scleroderma (P less than 0.05). The data suggest an ongoing activation of immune cells, particularly the CD4+ subset in SSc and indicate a potential role for the released mediator TNF, IL-1 beta, and lymphotoxin in the disease process.
为研究免疫机制在硬皮病（系统性硬化症，SSc）中的作用，我们检测了患者血清中特定细胞因子和可溶性免疫标志物的水平。本报告以42例患者和14例匹配的健康对照为研究对象。在SSc组中，42例中有8例检测到肿瘤坏死因子（TNF）（平均水平±标准差为29±539 pg/ml），42例中有36例检测到淋巴毒素（血清稀释度为1:409 - 1:200）。42例中有23例检测到白细胞介素β（IL-1β）（44±29 U/ml）。42例中有36例患者检测到IL-2，平均水平为286±406 U/ml，42例均检测到可溶性白细胞介素-2受体（1055±393 U/ml），42例中有27例检测到可溶性CD4抗原（血清稀释度为1:10 - 1:320），42例均检测到CD8（470±134 U/ml）。对照组未检测到TNF、淋巴毒素、IL-1β、IL-2和CD4。对照组的IL-2受体水平为520±171 U/ml，显著低于硬皮病患者（P<0.001），CD8水平（582±140）显著高于硬皮病患者（P<0.05）。数据表明免疫细胞持续激活，尤其是SSc中的CD4+亚群，并提示释放的介质TNF、IL-1β和淋巴毒素在疾病过程中可能发挥作用。
