Hubbard R D, Collins F M
Trudeau Institute, Inc., Saranac Lake, New York 12983.
Infect Immun. 1991 Feb;59(2):570-4. doi: 10.1128/iai.59.2.570-574.1991.
When C57BL/6 mice were infected intravenously with Mycobacterium avium, bacterial growth continued within the spleen until more than 10(8) CFU/g of tissue were attained. This contrasted with Mycobacterium bovis BCG infections where growth declined after 2 weeks. In vivo M. avium-infected splenic macrophages were harvested from chronically infected mice and cultured in vitro for 4 days at 37 degrees C. The number of viable mycobacteria within the resulting macrophage monolayers decreased when cultured in the presence of autologous sensitized T cells and an exogenous source of interleukin-2 (recombinant interleukin-2; 50 U/ml) compared with untreated controls (P less than 0.05). Incubation of the infected macrophages with autologous T cells and soluble M. avium antigens also significantly reduced the number of viable organisms. These results indicate that the mycobactericidal activity of M. avium-infected macrophages can be enhanced in a way that may have important therapeutic implications for patients infected with this opportunistic pathogen.
当C57BL/6小鼠经静脉注射感染鸟分枝杆菌后，细菌在脾脏内持续生长，直至组织中每克达到超过10(8) 菌落形成单位（CFU）。这与牛分枝杆菌卡介苗（Mycobacterium bovis BCG）感染形成对比，后者在2周后生长下降。从慢性感染小鼠中获取体内感染鸟分枝杆菌的脾巨噬细胞，并于37℃体外培养4天。与未处理的对照相比，当在自体致敏T细胞和白细胞介素-2外源来源（重组白细胞介素-2；50 U/ml）存在的情况下培养时，所得巨噬细胞单层内活的分枝杆菌数量减少（P<0.05）。将感染的巨噬细胞与自体T细胞和可溶性鸟分枝杆菌抗原一起孵育也显著减少了活的生物体数量。这些结果表明，感染鸟分枝杆菌的巨噬细胞的杀分枝杆菌活性可以通过一种可能对感染这种机会性病原体的患者具有重要治疗意义的方式得到增强。
