Department of Chemistry and Biochemistry, Institute of Biosciences, São Paulo State University, UNESP, 18618-000, Botucatu, São Paulo, Brazil.
Evid Based Complement Alternat Med. 2011;2011:643269. doi: 10.1093/ecam/nen070. Epub 2011 Feb 20.
This study was designed to determine whether N-acetylcysteine (NAC, C(5)H(9)-NO(3)S), a compound from Allium species may be used as a complementary therapeutic agent, to inhibit high-sucrose induced-obesity and its effects on glucose tolerance, in vivo low-density lipoprotein (LDL)-oxidation and serum oxidative stress in rats. Initially, 24 male Wistar rats were divided into two groups: controls receiving standard chow (C, n = 6) and those receiving high-sucrose diet (HS, n = 18). After 22 days, (HS) group was divided into three groups (n = 6/group); (HS-HS) continued to eat high-sucrose diet and water; (HS-N) continued to eat high-sucrose diet and received 2 mg l(-1)-NAC in its drinking water; (HS-CN) changing high-sucrose to standard chow and receiving 2 mg l(-1)-NAC in its drinking water. After 22 days of the HS-group division (44 days of experimental period) body weight, body mass index and surface area were enhanced in HS-HS rats (P < .001). HS-HS rats had glucose intolerance, increased serum triacylglycerol (TG), very low-density lipoprotein (VLDL), oxidized-LDL (ox-LDL) and lipid-hydroperoxide (LH) than the others (P < .01). NAC in HS-N and HS-CN rats reduced the obesity markers, feed efficiency, LH and ox-LDL, as well normalized glucose response, TG and VLDL (P < .01) in these groups compared with HS-HS. Total antioxidant substances, GSH/GSSG ratio and glutathione-reductase, were higher in HS-N than in HS-HS (P < .01). In conclusion, NAC improved high-sucrose diet-induced obesity and its effects on glucose tolerance, lipid profile, in vivo LDL-oxidation and serum oxidative stress, enhancing antioxidant defences. The application of this agent may be feasible and beneficial for high-sucrose diet-induced obesity, which certainly would bring new insights on obesity-related adverse effects control.
本研究旨在确定 N-乙酰半胱氨酸（NAC，C(5)H(9)-NO(3)S），一种来自葱属植物的化合物，是否可以作为一种补充治疗药物，抑制高蔗糖诱导的肥胖及其对葡萄糖耐量、体内低密度脂蛋白（LDL）氧化和血清氧化应激的影响。最初，将 24 只雄性 Wistar 大鼠分为两组：对照组（C）接受标准饲料（n = 6），高蔗糖组（HS）接受高蔗糖饮食（n = 18）。22 天后，（HS）组分为三组（n = 6/组）：（HS-HS）继续食用高蔗糖饮食和水；（HS-N）继续食用高蔗糖饮食并饮用 2mg/L-1 NAC；（HS-CN）将高蔗糖饮食改为标准饮食并饮用 2mg/L-1 NAC。HS 组分组后 22 天（实验期 44 天），HS-HS 大鼠体重、体重指数和表面积增加（P<0.001）。与其他组相比，HS-HS 大鼠糖耐量降低，血清三酰甘油（TG）、极低密度脂蛋白（VLDL）、氧化 LDL（ox-LDL）和脂质过氧化物（LH）升高（P<0.01）。HS-N 和 HS-CN 组大鼠的 NAC 降低了肥胖标志物、饲料效率、LH 和 ox-LDL，并使这些组的葡萄糖反应、TG 和 VLDL 正常化（P<0.01）。与 HS-HS 相比，HS-N 组的总抗氧化物质、GSH/GSSG 比值和谷胱甘肽还原酶较高（P<0.01）。综上所述，NAC 改善了高蔗糖饮食诱导的肥胖及其对葡萄糖耐量、脂质谱、体内 LDL 氧化和血清氧化应激的影响，增强了抗氧化防御能力。该药物的应用可能是可行和有益的，用于高蔗糖饮食诱导的肥胖，这无疑将为肥胖相关不良反应的控制带来新的见解。
