Lovett P S, Ambulos N P, Mulbry W, Noguchi N, Rogers E J
Department of Biological Sciences, University of Maryland-Baltimore County, Catonsville 21228-5398.
J Bacteriol. 1991 Mar;173(5):1810-2. doi: 10.1128/jb.173.5.1810-1812.1991.
Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.
将cat-86基因的第7或144位密码子替换为UGA密码子，可使该基因赋予野生型枯草芽孢杆菌氯霉素抗性。相同密码子替换为UAA后，在野生型枯草芽孢杆菌中产生氯霉素敏感表型，而在抑制子阳性菌株中产生氯霉素抗性表型。N端测序表明，野生型细胞中第7位密码子的UGA被解码为色氨酸，解码效率约为6%。
