Lipsky R H, Rosenthal R, Zahler S A
J Bacteriol. 1981 Dec;148(3):1012-5. doi: 10.1128/jb.148.3.1012-1015.1981.
We isolated defective specialized transducing phages of SP beta that carry one of the extracistronic suppressors, sup-3 or sup-44. Lysates containing these phages can be used in a simple spot test to determine whether an auxotrophic mutation can be suppressed. The sup-3 and sup-44 mutations are distinct, in that their suppression patterns differ for the markers hisA1, metC3, and thr-5; and they are not alleles.
我们分离出了携带顺反子外抑制基因sup-3或sup-44之一的SPβ缺陷型特异性转导噬菌体。含有这些噬菌体的裂解物可用于一项简单的斑点试验,以确定营养缺陷型突变是否能够被抑制。sup-3和sup-44突变是不同的,因为它们对hisA1、metC3和thr-5这些标记的抑制模式不同;并且它们不是等位基因。