毛诃子变种薄叶变种提取物对丝裂霉素 C 诱导的人淋巴细胞染色体畸变和姐妹染色单体交换的影响。
Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes.
机构信息
Science Faculty, Department of Biology, Kahramanmaraş Sütcü Imam University, 46100, Kahramanmaraş, Türkiye.
出版信息
Cytotechnology. 2006 Jun;51(2):99-104. doi: 10.1007/s10616-006-9015-6. Epub 2006 Sep 21.
In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 mug ml(-1)) plus 10(-5), 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10(-2) mul ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10(-4), 10(-3) and 10(-2) mul ml(-1) concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.
在这项研究中，我们评估了 Thymus kotschyanus var. glabrescens Boiss. 提取物（TE）的致裂效应和该提取物对丝裂霉素 C（MMC）诱导的染色体损伤的抗致裂效应，采用人外周血淋巴细胞体外实验。进行了两个系列的实验。在第一个实验中，仅使用 10(-5)、10(-4)、10(-3)和 10(-2)mul ml(-1)浓度的 TE 处理 48 小时，以检测潜在的致裂性。在第二个实验中，使用 MMC（0.38 mug ml(-1))和 10(-5)、10(-4)、10(-3)和 10(-2)mul ml(-1)浓度的 TE 处理 48 小时，以确定抗致裂效应。与阴性和溶剂对照组相比，TE 并没有显著增加姐妹染色单体交换（SCEs）（除 10(-2)mul ml 浓度外）和染色体畸变（CAs）的频率。然而，它降低了 MMC 诱导的染色体畸变的频率。在 10(-4)、10(-3)和 10(-2)mul ml(-1)浓度下降低效果显著。另一方面，与阳性对照组相比，TE 显著增加了所有处理组中 MMC 诱导的 SCEs。
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