School of Pure and Applied Natural Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden.
Cytotechnology. 2007 Sep;55(1):3-8. doi: 10.1007/s10616-007-9097-9. Epub 2007 Oct 11.
A novel supplementation of cell growth media based on a porcine platelet lysate was developed for culture of animal-derived cells. The platelet lysate was produced from porcine blood and contained lysate of platelets and plasma components. It showed satisfactory microbiological integrity and it carried only low amount of endotoxins (<10 EU/mL). The porcine platelet lysate supported well proliferation of Vero (African green monkey transformed kidney epithelial cells), Chinese hamster ovary (CHO) and hybridoma cells comparable to fetal bovine serum (FBS). Platelet lysate shows promise as a viable choice over FBS as it can be produced in large quantities, high lot-to-lot consistency and with an attractive price structure. Furthermore it is a strong alternative to FBS for ethical reasons. It is expected that it can be used as a general supplementation for most animal cells for research studies on the proliferation of cells and their expression of products.
开发了一种基于猪血小板裂解液的新型细胞生长培养基补充剂，用于培养动物源性细胞。血小板裂解液由猪血液制成，包含血小板和血浆成分的裂解液。它具有令人满意的微生物完整性，并且仅携带低量的内毒素（<10 EU/mL）。猪血小板裂解液支持 Vero（非洲绿猴转化的肾上皮细胞）、中国仓鼠卵巢（CHO）和杂交瘤细胞的增殖，与胎牛血清（FBS）相当。血小板裂解液有望成为 FBS 的可行替代品，因为它可以大量生产，具有高批次间一致性和有吸引力的价格结构。此外，出于伦理原因，它也是 FBS 的有力替代品。预计它可以用作大多数动物细胞的一般补充剂，用于研究细胞增殖及其产物表达。
