使用光学传感器监测哺乳动物细胞培养中的 pH 值和溶解氧。
Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors.
机构信息
School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin, 4, Ireland,
出版信息
Cytotechnology. 2008 Jul;57(3):245-50. doi: 10.1007/s10616-008-9160-1. Epub 2008 Sep 19.
Abstract
Here, we have studied two parameters critical to process control in mammalian cell culture; dissolved oxygen (dO(2)) and pH, measured with fluorescent sensors thus allowing the study of the metabolic state of cells in culture without removing or damaging cells during cultivation. Two cell lines, namely, NS0 and CHO were batch-grown in 24-well plates at different serum concentrations with the sensors implemented in the bottom of each well. The data showed a good relationship between the dO(2) and pH data obtained from fluorescent probes and the growth and death characteristics of cells. The method has provided a high throughput on-line multi-parametric analysis of mammalian cell cellular activity.
摘要
在这里，我们研究了两个对哺乳动物细胞培养过程控制至关重要的参数;溶解氧(dO(2))和 pH 值，使用荧光传感器进行测量，从而可以在不取出或损坏细胞的情况下研究培养过程中细胞的代谢状态。我们使用荧光探针，在不同血清浓度下，将 NS0 和 CHO 两种细胞系在 24 孔板中进行分批培养，并将传感器安装在每个孔的底部。实验数据表明，荧光探针获得的 dO(2)和 pH 值数据与细胞的生长和死亡特征之间存在良好的相关性。该方法提供了一种高通量的在线多参数分析哺乳动物细胞活性的方法。
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