Instituto de la Grasa (Consejo Superior de Investigaciones Científicas), Avenida Padre García Tejero, 4, 41012, Sevilla, Spain,
Cytotechnology. 2008 Jul;57(3):263-72. doi: 10.1007/s10616-008-9170-z. Epub 2008 Oct 7.
The growth of mammalian cells in vitro requires the use of rich culture media that are prepared by combining serum with specific nutrient formulations. Serum, the most expensive component of culture media, provides a complex mixture of growth factors and nutrients. Protein hydrolysates that can support in vitro cell growth and eliminate or reduce the need to use serum have been obtained from different sources. Here we describe the use of two food grade proteases to produce a chickpea protein hydrolysate that has been added to cell culture medium in order to determine whether it can be used as a substitute for serum. Medium containing the hydrolysate has been tested using two human cells lines: the monocytic THP-1 cell line which grows in suspension, and the epithelial Caco-2 cell line which grows as a monolayer. The chickpea protein hydrolysate was a good substitute for serum in the first case, but did not allow growth of Caco-2 cells. Supplementation of culture media with this inexpensive and safe hydrolysate would greatly reduce the cost of cell culture.
哺乳动物细胞的体外生长需要使用富含血清的培养基，该培养基由血清与特定的营养配方结合而成。血清是培养基中最昂贵的成分，提供了复杂的生长因子和营养混合物。已经从不同来源获得了可支持体外细胞生长并消除或减少使用血清需求的蛋白质水解物。在这里，我们描述了使用两种食品级蛋白酶来生产鹰嘴豆蛋白水解物，该水解物已添加到细胞培养基中，以确定它是否可以用作血清的替代品。已经使用两种人细胞系测试了含有水解物的培养基：悬浮生长的单核细胞 THP-1 细胞系和作为单层生长的上皮 Caco-2 细胞系。在第一种情况下，鹰嘴豆蛋白水解物是血清的良好替代品，但不能使 Caco-2 细胞生长。用这种廉价且安全的水解物补充培养基将大大降低细胞培养的成本。
