Department of Applied Biological Science, Tokyo Noko University, Saiwaicho 3-5-8, Fuchu, Tokyo, 183-8509, Japan.
Cytotechnology. 2001 Jul;36(1-3):187-93. doi: 10.1023/A:1014008129116.
The bioavailability and action of teas on the invasion of a rat ascites hepatoma cell line, AH109A, were determined and their modes of action were by co-culturing the cancer cells with a rat mesentery-derived mesothelial-cell (M-cell) monolayer in the presence of sera from rats orally given teas and their component, (-)-epigallocatechin gallate (EGCG). The rat sera obtained 2 and 5 hr after oral intubation of a low concentration of green, oolong, or black tea, or EGCG significantly inhibited AH109A invasion underneath the M-cell monolayer. These sera showed a time-dependent and significant inhibitory effect on the AH109A invasion. The 2-hr sera and 2.5 muM EDTA in the medium completely eliminated the enhancement of AH109A invasion induced by a reactive oxygen species (ROS)-generating system. These results show that the inhibition of relevant ROS-potentiated invasion of AH109A cells across the M-cell monolayer may be due to the antioxidative action of EGCG, the in vivo metabolites, and tea-induced changes in the endogenous substances. The results suggest that the drinking of tea in daily life may have certain preventive and therapeutic effects against cancer cell invasion.
茶水对大鼠腹水肝癌细胞系 AH109A 侵袭的生物利用度和作用进行了测定，并通过在含有来自口服给予茶水及其成分（-）-表没食子儿茶素没食子酸酯（EGCG）的大鼠血清的情况下，将癌细胞与大鼠肠系膜衍生的间皮细胞（M 细胞）单层共培养来研究其作用方式。口服低浓度绿茶、乌龙茶或红茶或 EGCG 后 2 小时和 5 小时获得的大鼠血清显著抑制了 AH109A 在 M 细胞单层下的侵袭。这些血清对 AH109A 的侵袭具有时间依赖性和显著的抑制作用。培养基中的 2 小时血清和 2.5 μM EDTA 完全消除了由活性氧（ROS）生成系统诱导的 AH109A 侵袭的增强。这些结果表明，相关 ROS 增强的 AH109A 细胞穿过 M 细胞单层的侵袭的抑制可能归因于 EGCG、体内代谢物和茶诱导的内源性物质变化的抗氧化作用。结果表明，日常生活中饮茶可能对癌细胞侵袭具有一定的预防和治疗作用。
