Department of Applied Biological Science, Tokyo Noko University, Fuchu, Tokyo, 183-8509, Japan.
Cytotechnology. 2001 Jan;35(1):57-63. doi: 10.1023/A:1008167502110.
Curcumin, a yellow pigment in turmeric, is a food factor withantioxidative activity. The effect of curcumin on the proliferation and invasion of the rat ascites hepatoma AH109Acells was studied in vitro and ex vivo assay systems. Especially, a co-culture system of the hepatoma cellswith mesothelial cells derived from rat mesentery was employed to investigate the invasive motility. Curcumin suppressed thehepatoma slipping motility in a dose-dependent manner up to 5 muM and thereafter maintained the effect up to 20 muM, whereas this substance exerted little influence on the proliferation of the hepatoma cells at the same concentrations. Sera obtained from rats orally given curcumin also inhibited the AH109A cellular invasive movement when added to the culturemedium. Hepatoma cells previously cultured with hypoxanthineand xanthine oxidase showed a highly invasive activity. Curcumin and curcumin-loaded rat sera suppressed this reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with hypoxanthine, xanthine oxidase and either of curcumin samples. These resultssuggest that the antioxidative property of curcumin may beinvolved in its anti-invasive action.
姜黄色素是姜黄中的一种黄色素，具有抗氧化活性，是一种食品因子。本文研究了姜黄素对体外和体内大鼠腹水肝癌 AH109A 细胞增殖和侵袭的影响。特别采用肝癌细胞与大鼠肠系膜间皮细胞的共培养系统来研究侵袭迁移能力。姜黄素以 5μM 为界，呈剂量依赖性抑制肝癌滑动运动，5μM 以上浓度可维持该抑制效果，而在相同浓度下，该物质对肝癌细胞的增殖影响甚微。口服给予姜黄素的大鼠血清也可抑制加入培养基中的 AH109A 细胞的侵袭运动。先前用次黄嘌呤和黄嘌呤氧化酶培养的肝癌细胞具有高度侵袭活性。姜黄素和负载姜黄素的大鼠血清可通过同时用次黄嘌呤、黄嘌呤氧化酶和姜黄素处理 AH109A 细胞来抑制这种活性氧增强的侵袭能力。这些结果表明，姜黄素的抗氧化特性可能与其抗侵袭作用有关。
